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Plasmid#87379PurposeRetroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).DepositorInsertTXN2 mitochondrial transit peptide (TXN2 Human)
UseRetroviralTagsDsRed1ExpressionMammalianMutationFirst 60 aa onlyPromoterRetroviral 5' LTR promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
modRNAc1-VP64dCas9VP64-2A-MPH
Plasmid#216388Purposethis vector can be used to make modRNA expressing VP64dCas9VP64 and MPHDepositorInsertVP64dCas9VP64-2A-MPH
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 CB1
Plasmid#13391PurposeExpresses mouse cannabinoid receptor 1 (CB1) in mammalian cellsDepositorInsertCB1 (Cnr1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFP.E227
Plasmid#138249PurposeReporter plasmid - Expresses three fluorescent reporters from three different ECF promotersDepositorInsertsmCherry
Terminator
mTagBFP
Terminator
gfp
Terminator
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP17_up1691, P20_992, and iP16_3622Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS133
Plasmid#211504PurposeAAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertmTagBF2
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
His6-pAG-RTase
Plasmid#214932PurposeExpress fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systemsDepositorInsertfusion protein of protein A/G and MMLV reverse transcriptase (25-497)
UseTagsHis6ExpressionBacterialMutationMMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…PromoterT7 promoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-c-Myc
Plasmid#13375PurposeRetroviral expression of mouse c-MycDepositorInsertmyelocytomatosis oncogene (Myc Mouse)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)
Plasmid#48313DepositorTypeEmpty backboneUseTagsHis6 and SumoExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pMRX-IB-TMEM192-mKeima
Plasmid#215359PurposeProbe to evaluate lysophagic activityDepositorInserttransmembrane protein 192 (TMEM192 Human)
UseRetroviralTagsmKeimaExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MS2_GFP
Plasmid#61764PurposeExpresses MS2 coat protein fused to GFPDepositorInsertMS2 coat protein fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationPromoterUbc promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV: Flag-Akaluc_SV40:PuroR
Plasmid#183048PurposeExpresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.DepositorInsertAkaLuc
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
T-2A-EGFP-PGK-Puro
Plasmid#83344PurposeDonor vector to knocin EGFP into human Brachyury(T) locusDepositorInsertHuman Brachyury knockin homology arms (TBXT Human)
UseTags2A-EGFPExpressionMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Stat2 pBluescript SK (-)
Plasmid#8705DepositorInsertStat2 (STAT2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Lifeact-tdTomato
Plasmid#64048PurposeFluorescent reporter for F-actin labeling in living cellsDepositorInsertLifeact
UseLentiviralTagstdTomatoExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG148 FRB-NFAST
Plasmid#130812PurposeExpresses FRB-NFAST in mammalian cellsDepositorInsertFRB-NFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_CasRx_GFP
Plasmid#134843PurposeAll in one overexpression of CasRx and guideRNADepositorInsertRfxCas13d
UseCRISPRTagsT2A EGFPExpressionMammalianMutationPromoterCAGAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
BiP-mGFP
Plasmid#62231PurposeEncodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.DepositorInserthamster BiP-mGFP-KDEL
UseTagsmGFPExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-HA-eLACCO2.1-NGR
Plasmid#208096PurposeBiosensor for extracellular L-lactateDepositorInserteLACCO2.1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-LMP1-IRES-mCherry
Plasmid#220351PurposeExpresses EBV latent gene LMP1 in mammalian cellsDepositorInsertLMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only