Addgene

We narrowed to 20,574 results for: crispr

Showing: 8841 - 8860 of 20574 results

Results per page:

U6-TM2Emp-6T_iBlue-ModFlanks

Plasmid

#200236

Purpose

Backbone for cloning longer RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Landing_Pad_8xCTS-ECFP-pA

Plasmid

#200242

Purpose

Backbone for cloning 8xCTSs (8xCRISPR-target sites)

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#194715

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sites

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#194726

Purpose

CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1(D908A)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D908A

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
P3-Lenti-dCas9-Tet1-GFP

Plasmid

#190729

Purpose

Lentiviral construct to express spdCas9-Tet1-GFP.

Depositor

Maja Jagodic

Insert

dCas9-tet1

Use

CRISPR

Tags

SV40 NLS and T2A-EGFP

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

UbC

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN102

Plasmid

#220045

Purpose

Mammalian expression of type III-A crRNA and Cas6 crRNA-processing ribonuclease from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA; Cas6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN053

Plasmid

#220047

Purpose

Mammalian expression of type III-A crRNA from Streptococcus thermophilus within 5`UTR of mCherry transcript

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAsCpf1(TYCV)(BB) (pY211)

Plasmid

#89352

Purpose

Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CBh

Availability

Academic Institutions and Nonprofits only

Enlarge
pAsCpf1(TATV)(BB) (pY221)

Plasmid

#89354

Purpose

Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1 RVR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K548V/N552R

Promoter

CBh

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-iCas9-neo

Plasmid

#85400

Purpose

Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Insert

Flag-iCas9-P2A-GFP

Use

Lentiviral

Tags

Flag-iCas9-P2A-GFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK-caCas9-NatMX-Neut5L

Plasmid

#89576

Purpose

caCas9 integrating vector into the Neut5L locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM-Cas9HF1

Plasmid

#89961

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9HF1

Use

Tags

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

Plasmid

#89991

Purpose

Donor template for generation of SOX2-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

none

Availability

Academic Institutions and Nonprofits only

Enlarge
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

Plasmid

#85041

Purpose

This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

loxP-stop-loxP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ220 (AsCpf1)

Plasmid

#86208

Purpose

AsCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

AsCpf1

Use

Gateway compatible cpf1 vectors

Tags

5' and 3' NLS

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_KDM1A

Plasmid

#86260

Purpose

Donor vector for 3' FLAG tag of human KDM1A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KDM1A homology arms (KDM1A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_FOXP1

Plasmid

#86261

Purpose

Donor vector for 3' FLAG tag of human FOXP1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXP1 homology arms (FOXP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_KDM6A

Plasmid

#86265

Purpose

Donor vector for 3' FLAG tag of human KDM6A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KDM6A homology arms (KDM6A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_KAT7

Plasmid

#86266

Purpose

Donor vector for 3' FLAG tag of human KAT7

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KAT7 homology arms (KAT7 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_KLF11

Plasmid

#86269

Purpose

Donor vector for 3' FLAG tag of human KLF11

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KLF11 homology arms (KLF11 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge