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Plasmid#127897PurposeHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a GeneDepositorInsertHp1a (Cbx5 Mustard Weed, Mouse)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationInserting GFP AID 2A Puro into mouse 3' Hp1aPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pDNR-gRNA
Plasmid#206990PurposeA plasmid for expression of SaCas9-sgRNA targeting donor plasmidsDepositorInsertDonor plasmid-targeting SaCas9-gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M2
Plasmid#135583PurposeHigh eukaryotic protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-M2
Plasmid#135584PurposeHigh eukaryotic dual protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS2
Plasmid#196978PurposeExpresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS2 tag
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1deltaTMD
Plasmid#108132PurposeRecruitable cytosolic SAC1DepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-521)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS1
Plasmid#196977PurposeExpresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS1 tag
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas9 Null
Plasmid#198727PurposeExpresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.DepositorInsertCas9
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR-221-P3-P5
Plasmid#186350PurposeGateway donor vector with AttP3 AttP5 recombination sitesDepositorTypeEmpty backboneUseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV-Gal4DBD2xHighMag1
Plasmid#182023PurposeDonor plasmid for conversion of Gal4 line into ShineGal4 driversDepositorInsertGal4DBD2xHighMag1
UseDonor plasmidTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_2
Plasmid#127555PurposeDrosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
mKusabira Orange-C1 (mKO)
Plasmid#54834PurposeLocalization: C1 Cloning Vector, Excitation: 548, Emission: 561DepositorTypeEmpty backboneUseTagsmKusabira OrangeExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion Halo-mAID
Plasmid#112852PurposeGeneral donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous proteinDepositorInsertHalo-mAID
UseSynthetic BiologyTagsExpressionMutationPromoterNoAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTetO-hOSK-CASH-1
Plasmid#188977PurposeA CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.DepositorInsertDoxycycline inducible reprogramming donor cassette
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
SAC1deltaTMD-FKBP-mCherry
Plasmid#108123PurposeRecruitable cytosolic SAC1DepositorInsertSACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-520)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1
Plasmid#108127PurposeRecruitable SAC1DepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L (SACM1L Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR-221-P3-P4
Plasmid#186351PurposeGateway donor vector with AttP3 AttP4 recombination sitesDepositorTypeEmpty backboneUseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only