Addgene

We narrowed to 14,637 results for: jun

Showing: 8761 - 8780 of 14637 results

Results per page:

Sort results by:

pT2EFCRET(#324)

Plasmid

#184059

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Tags

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Xl-EF1a

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SHIP1-SH2

Plasmid

#46492

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHIP1 (INPP5D Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-118

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_UdgX-EE-UdgX-nCas9-UdgX

Plasmid

#163566

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-nCas9-UdgX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pS6-TGFB2-STREP

Plasmid

#128503

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Use

Tags

STREP (2X) tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KAT2A sgRNA2

Plasmid

#138186

Purpose

3rd generation lentiviral gRNA plasmid targeting human KAT2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

KAT2A (KAT2A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
KAT2A sgRNA1

Plasmid

#138185

Purpose

3rd generation lentiviral gRNA plasmid targeting human KAT2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

KAT2A (KAT2A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB-hCRY1-SNAP-CD4-bla

Plasmid

#179447

Purpose

Donor vector to knock in SNAPtag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

Plasmid

#128835

Purpose

Knock-in of TagBFP into mouse Esrrb locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tagBFP into Esrrb locus (Esrrb Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

Plasmid

#179430

Purpose

Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-HS4-mch (SH)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF alpha, pRSV

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Coff/Fon-bREACHes-EYFP

Plasmid

#137147

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Coff/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

Plasmid

#102595

Purpose

Lentivector to express STING V155M delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V155M delta 354

Promoter

SFFV

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT/TO/HIS/FLAG/HA-API5

Plasmid

#38074

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

API5 apoptosis inhibitor 5 (API5 Human)

Use

Tags

HIS/FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SHP-1(NC)-SH2

Plasmid

#46496

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-1(NC) (PTPN6 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-221

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_APOBEC1-UdgX-NG-nCas9

Plasmid

#163533

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

APOBEC1-UdgX-NG-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
G protein alpha-s(alpha3beta5/G226A)

Plasmid

#55798

Purpose

In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.

Depositor

Catherine Berlot

Article

Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

Insert

alpha-s (alpha3beta5/G226A) (Gnas Rat)

Use

Tags

internal EE epitope (residues 189-194 in alpha-s …

Expression

Mammalian

Mutation

N271K, K274D, R280K, T284D, I285T, G226A in alpha…

Promoter

CMV

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_RBMX-eA3A-UdgX-NG-nCas9

Plasmid

#163551

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-NG-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Promoter

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBullet-pm-c

Plasmid

#53078

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-pro-siRNA-V2-LMNA

Plasmid

#111088

Purpose

pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Insert

Lamin A/C (LMNA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

Plasmid

#184406

Purpose

yeast surface display of the SARS-CoV-2 Beta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

K417N+E484K+N501Y

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe_puro_DEST_Flag_GPR160

Plasmid

#45274

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

GPR160 (GPR160 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only