pET-pro-siRNA-V2-LMNA
(Plasmid
#111088)
-
PurposepET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepET-30a based pET-pro-siRNA-V2
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameLamin A/C
-
Alt nameLMNA
-
gRNA/shRNA sequenceLMNA
-
SpeciesH. sapiens (human)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor notes that screening of multiple colonies for correct insert may be required.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET-pro-siRNA-V2-LMNA was a gift from Linfeng HUANG (Addgene plasmid # 111088 ; http://n2t.net/addgene:111088 ; RRID:Addgene_111088) -
For your References section:
Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli. Kaur G, Cheung HC, Xu W, Wong JV, Chan FF, Li Y, McReynolds L, Huang L. Biotechnol Bioeng. 2018 Jun 5. doi: 10.1002/bit.26740. 10.1002/bit.26740 PubMed 29873060
Map uploaded by the depositor.
