Addgene

We narrowed to 9,065 results for: yeast

Showing: 8681 - 8700 of 9065 results

Results per page:

Sort results by:

pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson

Plasmid

#117662

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pOst1-pro-af(MUT1)-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJA104

Plasmid

#128520

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme I56T (LYZ Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXZX501

Plasmid

#169455

Purpose

pRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassette

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

gRNA for MATa

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZ-mouseGIRK2-eGFP-StII-6xHis

Plasmid

#124277

Purpose

Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Use

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

Promoter

AOX1

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH727-CEN-RLuc/staCFLuc

Plasmid

#38211

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

Plasmid

#176400

Purpose

Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

FRB-RGG-GFP-RGG-RGG

Use

Tags

FRB and GFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
natMX-ins7

Plasmid

#195044

Purpose

pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

NatR

Use

Yeast genomic targeting

Tags

tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…

Expression

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJS646

Plasmid

#183175

Purpose

Y2H vector compatible with pJS647 PYR1 insert

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
416GPD-FUS-YFP

Plasmid

#29594

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pXZX351

Plasmid

#169454

Purpose

pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

gRNA for MATα

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAN200

Plasmid

#129203

Purpose

yTRAP sensor of [PSI+], detects aggregation of Sup35

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35NM (SUP35 Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

N terminal and middle domains

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK267

Plasmid

#129272

Purpose

"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

NLS-VP16-ZF 43-8-NES

Use

Tags

6xHA

Expression

Yeast

Mutation

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
303Gal-FUS

Plasmid

#29614

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pUDE735

Plasmid

#103024

Purpose

expression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
YCplac33-NAT_MCART1

Plasmid

#140591

Purpose

Express MCART1 in S. cerevisiae

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)

Use

Tags

Expression

Yeast

Mutation

codon-optimized for expression in S. cerevisiae

Promoter

NDT1 promoter and 3'UTR

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

Plasmid

#124278

Purpose

Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Use

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

R201A

Promoter

AOX1

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
nuc-yHS1-M7A

Plasmid

#159167

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1-M7A

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Mutated Met 7 of the cyt b562 module of nuc-yHS1 …

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
423Gal-FUS-YFP

Plasmid

#29615

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pMXs_FLAG-FSF1

Plasmid

#110641

Purpose

Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

FSF1 (FSF1 Budding Yeast)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDE722

Plasmid

#103022

Purpose

expression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crHCAN1-4.S (CAN1 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge