Skip to main content
Addgene
Showing: 8681 - 8700 of 54836 results

  2. R620-M06-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417N/E484K/N501Y

    Plasmid
    #170192
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    3C-His8-SBP
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170192-plasmid-map-sequence-id-334252

    Enlarge

  4. pTM-RBDv2

    Plasmid
    #162785
    Purpose
    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Use
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Mutation
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162785-plasmid-map-sequence-id-333799

    Enlarge

  5. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Philip Cole
    Article
    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021
    Insert
    S. aureus Sortase A mutant
    Use
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231144-plasmid-map-sequence-id-460063

    Enlarge

  9. R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

    Plasmid
    #170203
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    3C-His8-SBP
    Expression
    Mammalian
    Mutation
    K417T, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170203-plasmid-map-sequence-id-334219

    Enlarge

  11. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

    Plasmid
    #170191
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    His6
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170191-plasmid-map-sequence-id-334254

    Enlarge

  15. pT3EF1aH-myr-Akt

    Plasmid
    #179909
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.
    Depositor
    Xin Chen
    Article
    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Tags
    HA and Myr
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    179909-plasmid-map-sequence-id-357764

    Enlarge

  17. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

    Enlarge

  18. pTripZ EV

    Plasmid
    #209071
    Purpose
    DOX-ON inducible expression vector that has a stuffer sequence after an LR reaction and used as empty expression vector control
    Depositor
    Matthew Oser
    Article
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Insert
    stuffer DNA sequence after LR recombination with pTripZ NEO DEST to be used as an empty vector control
    Use
    Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    209071-plasmid-map-sequence-id-412293

    Enlarge

  19. elavl3:SomaGCaMP7f

    Plasmid
    #158760
    Purpose
    Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP7
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    HuC promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158760-plasmid-map-sequence-id-310667

    Enlarge

Showing: 8681 - 8700 of 54836 results