Skip to main content
Addgene
Showing: 8681 - 8700 of 154707 results

  3. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

    Enlarge

  4. pAAV-nEF-Con/Fon-iC++-EYFP (AAV8)

    Viral Prep
    #137155-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-iC++-EYFP (#137155). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-iC++-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Cre and Flp-dependent)
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only

  6. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid
    #71390
    Purpose
    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.
    Depositor
    Thomas Cech
    Article
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Use
    Hr donor
    Tags
    FLAG-SNAP
    Expression
    Mutation
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71390-plasmid-map-sequence-id-128451

    Enlarge

  7. PB53x EF1-Dendra2-RPB1Amr

    Plasmid
    #81228
    Purpose
    expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system
    Depositor
    Timothée Lionnet
    Article
    Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1
    Insert
    Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
    Use
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    N792D alpha amanitin resistance mutation (Bartolo…
    Promoter
    EF1a
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    81228-plasmid-map-sequence-id-394051

    Enlarge

  8. pLX302 EGFP-V5

    Plasmid
    #141348
    Purpose
    Expresses V5 tagged GFP
    Depositor
    Kevin Janes
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    V5 tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    141348-plasmid-map-sequence-id-307212

    Enlarge

  12. mCherry-VASP-5

    Plasmid
    #55151
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    VASP
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A209T in VASP
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55151-plasmid-map-sequence-id-424687

    Enlarge

Showing: 8681 - 8700 of 154707 results