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pET21a(+)-Bst LF-6xHis

Plasmid

#159148

Purpose

expresses His-tagged large fragment of Bst polymerase

Depositor

Andrea Pauli

Article

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Insert

large fragment of Geobacillus stearothermophilus DNA polymerase I

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET21a(+)-Bst LF-6xHis

Plasmid

#159148

Purpose

expresses His-tagged large fragment of Bst polymerase

Depositor

Andrea Pauli

Article

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Insert

large fragment of Geobacillus stearothermophilus DNA polymerase I

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEF Synaptophysin-mRuby

Plasmid

#188980

Purpose

Synaptic buton marker

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Rat, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpo

Plasmid

#60662

Purpose

Expresses pCAG-Flpo

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpo

Plasmid

#60662

Purpose

Expresses pCAG-Flpo

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCW57.1_AOX-FLAG

Plasmid

#177984

Purpose

All-in-one dox-inducible expression of Alternative Oxidase

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

AOX

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Codon optimized cDNA for expression in human cells

Promoter

TRE

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HDAC2-H141A

Plasmid

#115346

Purpose

Expresses mouse HDAC2 cDNA with H141A point mutation

Depositor

Javier Gonzalez-Maeso

Article

Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

Insert

Histone deacetylase 2 (Hdac2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H141A

Promoter

CMV

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HDAC2-H141A

Plasmid

#115346

Purpose

Expresses mouse HDAC2 cDNA with H141A point mutation

Depositor

Javier Gonzalez-Maeso

Article

Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

Insert

Histone deacetylase 2 (Hdac2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H141A

Promoter

CMV

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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hPGK_V5-APEX2-GFP

Plasmid

#105285

Purpose

drives expression of V5-NES-APEX2-GFP from human PGK promotor

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

V5-APEX2-NES

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

human PGK promoter

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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hPGK_V5-APEX2-GFP

Plasmid

#105285

Purpose

drives expression of V5-NES-APEX2-GFP from human PGK promotor

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

V5-APEX2-NES

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

human PGK promoter

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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JD553

Plasmid

#160392

Purpose

Wheat GRF4-GIF1 chimera in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

wheat GRF4-GIF1 chimera

Use

Entry clon

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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JD553

Plasmid

#160392

Purpose

Wheat GRF4-GIF1 chimera in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

wheat GRF4-GIF1 chimera

Use

Entry clon

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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enIscB

Plasmid

#205410

Purpose

Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

Insert

pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry

Use

Tags

Expression

Mammalian

Mutation

E85R+H369R+S387R+S457R

Promoter

CAG, hU6, CMV

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-I-GFP

Plasmid

#1736

Purpose

This retroviral vector has an IRES-GFP downstream of the cloning sites

Depositor

Garry Nolan

Insert

IRES-GFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pBMN-I-GFP

Plasmid

#1736

Purpose

This retroviral vector has an IRES-GFP downstream of the cloning sites

Depositor

Garry Nolan

Insert

IRES-GFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-eGFP-PGK-mCherry

Plasmid

#191862

Purpose

Heat inducible eGFP reporter with constitutive mCherry marker

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

eGFP
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

HSPA7 promoter and PGK promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-GFP-CtIP

Plasmid

#71109

Purpose

mammalian expession of GFP-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCW-GFP-CtIP

Plasmid

#71109

Purpose

mammalian expession of GFP-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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DNA repair library with 1,573 sgRNAs

Pooled Library

#177663

Purpose

CRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processes

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Species

Homo sapiens

Use

CRISPR

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only
pLVX_DSB_Spectrum-V3

Plasmid

#192476

Purpose

Lentiviral DSB Spectrum V3 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, mCherry, incompleteGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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