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Plasmid#179633PurposeSEAP RELEASE construct with AAMP motif controlDepositorInsertSEAP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)
Plasmid#44083DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-p62
Plasmid#199780PurposeUsed for the expression of mCherry labelled p62 (SMC Internal No. 391).DepositorInsertp62 (NUP62 Human)
UseTags6x His, TEV, mCherryExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR
Plasmid#207355PurposeLentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertRNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV syn CCR6-eLOV-TEVcs-tTA
Plasmid#173116PurposeExpresses CCR6 receiver construct in neuronsDepositorInsertCCR6-eLOV-TEVcs-tTA
UseAAVTagsExpressionMutationPromoterSynapsinAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET Flag TEV LIC cloning vector (2L-T)
Plasmid#29715DepositorTypeEmpty backboneUseTagsFLAG-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII TEV LIC cloning vector (2R-T)
Plasmid#29717DepositorTypeEmpty backboneUseTagsStrepII-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2
Plasmid#105801PurposeConcomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: FLAG-3C; ORF2: mClover3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)
Plasmid#44095DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus
Plasmid#105805PurposeConcomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-Cerulean; ORF2: TEV-VenusExpressionMammalianMutationPromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)
Plasmid#139788PurposeVector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)
Plasmid#44094DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE
Plasmid#166798PurposeMammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.DepositorInsertDPP9 (DPP9 Human)
UseTagsFLAG-TEVExpressionMammalianMutationLL100EEPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-OPTN WT
Plasmid#190191PurposePlasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797DepositorInsertoptineurin (OPTN Human)
UseTags6xHis, TEV, and mCherryExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant
Plasmid#149507PurposeTransient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.DepositorInsertMFSD12 (MFSD12 Human)
UseTransient expressionTagsTEV-3XHA-3XFLAGExpressionMammalianMutationCodon Optimized Sequence, p.LL256-257AAPromoterCMVAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBS1551 H14-SUMO-TDP43 F-S-TEV-mCherry
Plasmid#133321PurposeBacterial expression of TDP43 with F-S mutant CTDDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionBacterialMutationAll F in the TDP43 IDRs mutated to SPromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTF264 (pFA6a-TEV-6xGly-3xFlag-His3MX)
Plasmid#44071DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
T7pr-His6-MBP-TEV-dCas9-BirA*
Plasmid#159994PurposeBacterial expression of dCas9-BirA*DepositorInsertdCas9-BirA*
UseTags6X-His, MBP, 3X-FLAG, NLSExpressionBacterialMutationD10A, H840APromoterT7Available sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-b2AR-hLOV1-TEVcs-FLAG-GAL4-V5
Plasmid#104842Purposeb2AR-SPARK TF componentDepositorInserthLOV1-TEVcs-FLAG-GAL4-V5
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)
Plasmid#174336PurposeThe ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2DepositorInserthuman Orai1 (ORAI1 Human)
UseRetroviralTagspHluorin inserted in the extracellular loop 2 of …ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only