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Plasmid#15269PurposeRetroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)DepositorInsertBRAF (BRAF Human)
UseRetroviralTagsHis and MycExpressionMammalianMutationValine 600 to Glutamate (mutation has been found …PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-dTAG
Plasmid#207683PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-dTAG cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag-FOXO4
Plasmid#153143PurposeExpression of Flag tagged human FOXO4 in mammalian cellsDepositorInserthuman FOXO4 (FOXO4 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mRuby2
Plasmid#175294PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mRuby2
UseTagsmRuby2ExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
SIRT3 Flag
Plasmid#13814PurposeMammalian expression of human SIRT3 with flag tagDepositorInsertSIRT3 (SIRT3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP22
Plasmid#22575DepositorInsertUSP22 (USP22 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-PS9
Plasmid#177944PurposeTransient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInserteGFP (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-OTULIN (full-length, aa 1-352)
Plasmid#61464PurposeExpresses human OTULIN (full-length) in E. coli.DepositorInsertOTULIN (OTULIN Human)
UseTagsHis6-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SOX2
Plasmid#26817DepositorInsert5'UTR-SOX2-3'UTR (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Ef1a-fDIO-tdTomato
Plasmid#128434PurposeExpresses tdTomato under control of eukaryotic Ef1a promoter after Flp-dependent recombinationDepositorHas ServiceAAV1InserttdTomato
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-SADB19P
Plasmid#32631DepositorInsertSADB19P
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR2-YFP
Plasmid#13016DepositorInsertToll-Like Receptor 2 (TLR2 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
iRFP-PH-PLCdelta1
Plasmid#66841Purposeexpression of near-infrared PI(4,5)P2 biosensorDepositorInsertPH-PLCdelta1
UseTagsiRFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI152-pLenti-SiT-Cas12a
Plasmid#128405PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro human IRS-1
Plasmid#11025DepositorInsertIRS-1 (IRS1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-MTS-EGFP
Plasmid#190270PurposeLentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cellsDepositorInsertMTS-EGFP
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_FURIN_WT
Plasmid#82122PurposeGateway Donor vector containing FURIN , part of the Target Accelerator Plasmid Collection.DepositorInsertFURIN (FURIN Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
SpyTag003-mKate2
Plasmid#133452PurposeExpresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mKate2
UseTagsGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only