Showing: 8521 - 8540 of 147675 results
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Plasmid#67184PurposeConjugative cloning vectorDepositorTypeEmpty backboneUseConjugative bacterial cloning vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
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pNUT N6His hTFNG
Plasmid#67240PurposeExpresses N-His tagged nonglycosylated human serum transferrin in mammalian cellsDepositorInserthuman serum transferrin (TF Human)
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationAsn413 Asp, Asn611AspPromoterSV40AvailabilityAcademic Institutions and Nonprofits only -
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TbSIR2RP1
Plasmid#67242PurposeDetermine its activity and specificityDepositorInsertTbSIR2RP1
UseTagsMBP, TEV, Thrombin, HIS, and noneExpressionBacterialMutationPromoterp-lacAvailabilityAcademic Institutions and Nonprofits only -
pMJH46
Plasmid#67272PurposeConjugally transferable red recombinogenic plasmidDepositorInserttraJ, cat, oriT
UseRed recombinaseTagsExpressionMutationPromotercat gene promoter from PGNS-BACAvailabilityAcademic Institutions and Nonprofits only -
pMJH65
Plasmid#67273PurposeConjugally transferable red recombinogenic plasmidDepositorInserttetA
UseRed recombinase, temperature sensitiveTagsExpressionBacterialMutationPromotertetA promoter of plasmid pACYC184 originAvailabilityAcademic Institutions and Nonprofits only -
pCMT-flp
Plasmid#67274PurposeConjugally transferable Flp recombinase plasmid for unmarked mutagenesisDepositorInserttetA, flp, repA101, oriR101
UseFlp recombinase plasmid, temperature sensitiveTagsHis-SUMO (flp)ExpressionMutationPromotertetA promoter of plasmid pACYC184 originAvailabilityAcademic Institutions and Nonprofits only -
Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR
Plasmid#67275PurposeFor sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell linesDepositorInsertpuromycin resistant gene
UseTagslinked to red flouresent protein gene (katushka)ExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3
Plasmid#67276PurposeRMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PADepositorInsertsGFP (mWasabi)
hygro
UseTagsF3 and FrtExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
Plasmid#67277Purposeinducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.DepositorInsertsfirst casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
UseTagsmTQ2 blue fluorescent and red flourescent gene ka…ExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
Villinpromoter-blue-FlpOERT2
Plasmid#67278Purpose4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertmTQ2=mturquoise2 blue fluorescent gene
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMutationPromoterVillinAvailabilityAcademic Institutions and Nonprofits only -
pdx1-mTQ2-P2A-FlpO-PA
Plasmid#67279PurposePancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertsmTQ2=mturquoise2 blue fluorescent gene
FlpO recombinase
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMammalianMutationPromoterpdxAvailabilityAcademic Institutions and Nonprofits only -
pUNC2001
Plasmid#67280PurposeExpresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcwDepositorInsertVibrio cholera photolyase
UseTagsmaltose binding proteinExpressionBacterialMutationnone knownPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMalAnPL
Plasmid#67281PurposeExpresses MBP-Anacystis nidulans photolyase in E. coliDepositorInsertphotolyase Anacystis nidulans
UseTagsmaltose binding proteinExpressionBacterialMutationnone knownPromoterAvailabilityAcademic Institutions and Nonprofits only -
pfbiRS
Plasmid#67283PurposeExpresses the fbiC gene from Mycobacterium smegmatisDepositorInsertfbiC gene from Mycobacterium smegmatis
UseTagspolyhistidineExpressionMammalianMutationnon knownPromoterAvailabilityAcademic Institutions and Nonprofits only -
p64PLA5
Plasmid#67284Purpose6/4 photolyase gene from Drosophila in pFastbac vector, intermediate step in insect cell expressionDepositorInsert6/4 photolyase from Drosophila
UseIntermediate for insect expressionTagsFlagExpressionMutationnone knownPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFBCPDL6
Plasmid#67285PurposeCPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expressionDepositorInsertCPD photolyase (phr Fly)
UseBacterial vector, insert not expressedTagsFlagExpressionMutationV275E*PromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-TM0848
Plasmid#67287PurposeExpresses TM0848 in E. coliDepositorInsertGTP-binding protein YqeH
UseTagsN tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Ascl1.MIRT124_x4 Xma_lost.WPRE
Plasmid#67290PurposeInduce human iN conversion from fibroblastDepositorInsertAsc1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE
Plasmid#67291PurposeInduce human iN conversion from fibroblastDepositorInsertAsc1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailabilityAcademic Institutions and Nonprofits only
Showing: 8521 - 8540 of 147675 results