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Plasmid#205753PurposeExpression of rsEGFP2-E in mamalian cellsDepositorInsertrsEGFP2-E
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Irf8
Plasmid#139838Purposedoxycycline-inducible expression of mouse Irf8 in mammalian cellsDepositorInsertIrf8 (Irf8 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE hBRM
Plasmid#1961DepositorInsertBRM (SMARCA2 Human)
UseRetroviralTagsflagExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-BsaI_pJMP2776
Plasmid#208890PurposeAcinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pFRT-DHX36iso1
Plasmid#159585PurposeExpress N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-HyPer7
Plasmid#136464PurposeMammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imagingDepositorInsertHyPer7
UseTagsLifeActExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1
Pooled Library#1000000100PurposeCRISPR gRNA knockout pooled library (version 1) for targeting the human genomeDepositorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57-RFP-P2A-MCS (Neo)
Plasmid#89182PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1
Plasmid#158738PurposeMammalian expression of scFLARE1 componentDepositorInsertscFLARE1
UseAAVTagsFlag, V5ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-FAK WT
Plasmid#186148PurposeFor expressing FAK in mammalian cellsDepositorInsertPTK2 (PTK2 Mouse)
UseTagsmEGFPExpressionMammalianMutationPromotercmvAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mIL2
Plasmid#74467Purposeexpression of mouse IL2 in mammalian cellsDepositorInsertIL2 (Il2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
Plasmid#61592PurposeA human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIK3CG
Plasmid#174189PurposeExpresses PIK3CG in mammalian cellsDepositorInsertPIK3CG (PIK3CG Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TrpRS-His
Plasmid#124120PurposeBacterial expression plasmid for PURE system componentsDepositorInsertTryptophanyl-tRNA ligase (trpS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDsRed-attP
Plasmid#51019PurposeVector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attPDepositorInsertsattP
LoxP-3xP3-DsRed-LoxP
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and NoneAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-eSpCas9(1.1)
Plasmid#108292PurposepX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only