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Plasmid#141309PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CTDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasinPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT deltaALAS
Plasmid#153471PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT deltaALASDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, deletion of amino aciā¦PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT delta24-36
Plasmid#153472PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT delta24-36DepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, deletion of amino aciā¦PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT E102A
Plasmid#153473PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT E102ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, E102APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT M122A
Plasmid#153474PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT M122ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, M122APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT G212A
Plasmid#153475PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT G212ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, G212APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
SaLgCG
Plasmid#164563PurposeGFPDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoterā¦Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
MPC026: pAAV-CAG-FLEX-NovArch-TS-Citrine_TSx3-ER2
Plasmid#153195Purposeencodes pAAV NovArchDepositorInsertNovArch
UseAAVTagscitrineExpressionMammalianMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAB-mOvol1
Plasmid#120360PurposepiggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).DepositorInsertOvol1 (Ovol1 Mouse)
UsePiggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-3
Plasmid#160703Purposetef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInserttef1-alpha
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 R14A/K310A
Plasmid#162655PurposeExpresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids R14 and K310 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-2xStrep-ORF68 C52A
Plasmid#162660PurposeLentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cellsDepositorInsertORF68
UseLentiviralTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid C52 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A
Plasmid#162639PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids K174, R179, and K182 mutated to alaniā¦PromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K435A
Plasmid#162651PurposeExpresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acid K435 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K450A/R451A
Plasmid#162653PurposeExpresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids K450 and R451 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-H112A RNAi-resistant
Plasmid#163621Purposemouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFPDepositorInsertmouse SNX27-H112A RNAi-resistant (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationH112A and RNAi resistancePromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8115 LentiCRISPR v2 sgPTPN14-3
Plasmid#163314PurposeExpresses Cas9 and a human PTPN14-targeting control guide RNADepositorInsertsgPTPN14-3
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shSpc25
Plasmid#160960PurposeSpc25 shRNA in pMKO.1 retroviral vectorDepositorInsertSpc25 shRNA (Spc25 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGAshort
Plasmid#153547PurposeUsed as a donor vector for the targeted correction of a mutation in PIGA exon 6DepositorInsertPIGA (a 603-bp fragment from intron 5 and exon 6)
UseDonor plasmidTagsN/AExpressionMutationNo mutationPromoterNo promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGAmut
Plasmid#153548PurposeUsed as a donor vector for the targeted knock-in of a mutation in PIGA exon 6DepositorInsertmutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
UseDonor plasmidTagsN/AExpressionMutationa 3-bp mutation in exon 6PromoterNo promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only