Addgene

We narrowed to 18,521 results for: omp

Showing: 8381 - 8400 of 18521 results

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pmScarlet-HSP70

Plasmid

#163790

Purpose

Expresses mScarlet-Hsp70 in mammalian cells

Depositor

Vincent Timmerman

Article

Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

Insert

HSP70 (HSPA1A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMIA4.721-Olônne

Plasmid

#214443

Purpose

BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMIA4.721-Keppel

Plasmid

#214444

Purpose

BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMIA4.721-Pansio

Plasmid

#214441

Purpose

BxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pENN226_CRISPRcharm

Plasmid

#220834

Purpose

CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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T7-Elongin C-pcDNA3

Plasmid

#19998

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

Elongin C (ELOC Human)

Use

Tags

T7-tag

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Puro-TNNT2-Neo

Plasmid

#214013

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_iRFP670 sgAAVS1

Plasmid

#192477

Purpose

All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Use

Tags

T2A iRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-EYFP-WPRE-bGHpA

Plasmid

#213971

Purpose

AAV expression of EYFP from pCALM1 promoter

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

pCALM1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_mCherry sg1-sg2

Plasmid

#192479

Purpose

All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Use

Tags

T2A mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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shTCF7L2 # 1

Plasmid

#42557

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTCF7L2 (TCF7L2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pSpCas9_T2A_iRFP670 sgBFP

Plasmid

#192478

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Use

Tags

T2A iRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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GPD-roGFP

Plasmid

#49436

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

GPD-roGFP2

Use

Adenoviral

Tags

Glycerol phosphate dehydrogenase, 5' to roGF…

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-eGFP

Plasmid

#193022

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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Matrix-roGFP

Plasmid

#49437

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

Matrix-roGFP2

Use

Adenoviral

Tags

Cytochrome c oxidase subunit IV mitochondrial tar…

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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lenti sgRNA(MS2)_puro backbone

Plasmid

#73795

Purpose

3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and EF1A

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_mCherry sgAAVS1

Plasmid

#192480

Purpose

All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Use

Tags

T2A mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-n1-APP

Plasmid

#69924

Purpose

mammalian expression of human APP fused to EGFP

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

APP (APP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDESTmycIGF2BP3

Plasmid

#19879

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pBRPB CAG-mCherry-IP

Plasmid

#106333

Purpose

PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

mCherry-IRES-Puro

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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