-
Plasmid#198651PurposeEasy-MISE toolkit pEM-plasmid containing PDA1 promoter with EF protruding endsDepositorInsertpPDA1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P05R
Plasmid#198650PurposeEasy-MISE toolkit pEM-plasmid containing CYC1 promoter with FG protruding endsDepositorInsertpCYC1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P05L
Plasmid#198649PurposeEasy-MISE toolkit pEM-plasmid containing CYC1 promoter with EF protruding endsDepositorInsertpCYC1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P04R
Plasmid#198648PurposeEasy-MISE toolkit pEM-plasmid containing TPI1 promoter with FG protruding endsDepositorInsertpTPI1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P04L
Plasmid#198647PurposeEasy-MISE toolkit pEM-plasmid containing TPI1 promoter with EF protruding endsDepositorInsertpTPI1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P03R
Plasmid#198646PurposeEasy-MISE toolkit pEM-plasmid containing PGK1 promoter with FG protruding endsDepositorInsertpPGK1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P03L
Plasmid#198645PurposeEasy-MISE toolkit pEM-plasmid containing PGK1 promoter with EF protruding endsDepositorInsertpPGK1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P02R
Plasmid#198644PurposeEasy-MISE toolkit pEM-plasmid containing ENO2 promoter with FG protruding endsDepositorInsertpENO2
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P02L
Plasmid#198643PurposeEasy-MISE toolkit pEM-plasmid containing ENO2 promoter with EF protruding endsDepositorInsertpENO2
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P01R
Plasmid#198642PurposeEasy-MISE toolkit pEM-plasmid containing TDH3 promoter with FG protruding endsDepositorInsertpTDH3
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P01L
Plasmid#198641PurposeEasy-MISE toolkit pEM-plasmid containing TDH3 promoter with EF protruding endsDepositorInsertpTDH3
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOAD eIF4G1 (1-341)
Plasmid#196833PurposeExpesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.DepositorInserteIF4G1 (TIF4631 Budding Yeast)
UseTagsGal4ExpressionYeastMutationamino acids 1-341PromoterADHAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2 Pab1 WT (123-204)
Plasmid#196834PurposeExpresses Pab1 RRM2 fragment fused to GAL4 DNA binding domainDepositorInsertPab1 (PAB1 Budding Yeast)
UseTagsGal4ExpressionYeastMutation123-204 amino acidsPromoterADHAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2 Pab1 E181R (123-204)
Plasmid#196835PurposeExpresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domainDepositorInsertPab1 (PAB1 Budding Yeast)
UseTagsGal4ExpressionYeastMutation123-204 amino acids & E181RPromoterADHAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCL.103
Plasmid#184988PurposeExpress Cas9-P2A-Eco1RTDepositorInsertCas9-P2A-Eco1RT
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.102
Plasmid#184987PurposeExpress -Eco1 RT-P2A-Cas9DepositorInsertEco1RT-P2A-Cas9
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM4_14
Plasmid#183062PurposePlasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHREsYGP1p and RET2Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2S
Plasmid#185865PurposeKnocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiaeDepositorInsertALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF71
Plasmid#185860PurposeTesting the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only