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Plasmid#215679PurposeEmpty repair plasmid for ChrIII split hygromycinR landing padDepositorInsert5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_ARPC1A:1-370
Plasmid#211194PurposeMammalian expression of full-length human ARPC1A. N-terminal HiBiT tag.DepositorInsertARPC1A:M1-M370 (ARPC1A Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmVen-Treslin
Plasmid#211405Purposemammalian expression of human Treslin N-terminally fused to mVenusDepositorInsertTreslin (C15orf42 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-B-eSpCas9-NLS-6xHis
Plasmid#207383PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-eSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060A, amino acids 1005-1013 repl…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)
Plasmid#185706PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLSDepositorInsertCas9
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30d-5p
Plasmid#103417PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30d-5p target (MIR30D Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
SMC6 G4.4 gRNA
Plasmid#90899Purpose3rd generation lentiviral gRNA plasmid targeting human SMC6DepositorInsertSMC6 (Guide Designation G4.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
SMC6 G3.4 gRNA
Plasmid#90898Purpose3rd generation lentiviral gRNA plasmid targeting human SMC6DepositorInsertSMC6 (Guide Designation G3.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV9)
Viral Prep#83896-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomato (physically separate, not a fusion protein)Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB-NES
Plasmid#61017PurposeddGFP B-NESDepositorInsertddGFP B
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
CN2109-rAAV-eHGT_410m-minBglobin-SYFP2-WPRE3-BGHpA
Plasmid#208157PurposeDirect-expressing SYFP2 in oligodendrocytes AAV VirusDepositorInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterBeta Globin minimal promoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCVhygro-Cre
Plasmid#34565DepositorInsertCre
UseRetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceMarch 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
β3-integrin-YFP
Plasmid#26653DepositorInsertβ3-integrin (ITGB3 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-NLS-mRuby2 (AAV9)
Viral Prep#99130-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDLX5/6TagsmRuby2Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJE3-E7
Plasmid#214359PurposeExpresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteinsDepositorInserts2nd generation Tet2 "E7" aminoacyl tRNA synthetase
Lpp promoted M. jannaschii tRNA
Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
Orthogonal "D4II" ColE1 origin
UseTagsExpressionBacterialMutationPromoterAmpR and lppAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 FS1
Plasmid#113955Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, one base pair dele…PromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.PI.EGFP.WPRE.bGH (AAV8)
Viral Prep#105530-AAV8PurposeReady-to-use AAV8 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsEGFPAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Tol2
Plasmid#31831DepositorInsertTol2 Transposase
UseCloning vectorTagsExpressionMutationPromoterLacAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
RCAS GFP (CT#22)
Plasmid#13878DepositorInsertGFP
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only