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150,426 results

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pMuc1

Plasmid

#73929

Purpose

Muconate expression plasmid. Expresses enzymes enabling production of muconate from glucose.

Depositor

George Church

Article

Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

Insert

muconate producing enzymes

Use

Tags

Expression

Mutation

Promoter

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pT3TS-nCas9n

Plasmid

#46757

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Bacterial

Mutation

Promoter

T3

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pKmK.P7

Plasmid

#125040

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

TEF1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16

Plasmid

#71728

Purpose

plasmid expressing fusion construct of Gal4 DBD and VP16 AD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD(1-147) (GAL4 Budding Yeast)

Use

Tags

VP16 AD (413-490)

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pExp_CAG:EYFP

Plasmid

#51791

Purpose

Constitutively expresses yellow fluorescent protein , EYFP

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

EYFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_BiVIPe4_dTomato_nlsdTomato

Plasmid

#213855

Purpose

AAV construct expressing dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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Factor IX donor

Plasmid

#182141

Purpose

Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald-Calnexin-C-14

Plasmid

#54020

Purpose

Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Calnexin (CANX Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Hygro DEST (w530-1)

Plasmid

#19066

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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SIRT2 Flag

Plasmid

#13813

Purpose

Mammalian expression of human SIRT2 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT2 (SIRT2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Transcript variant 2

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-his-BACH1 WT

Plasmid

#17642

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-Rbfox1

Plasmid

#213725

Purpose

Mammalian cell expression vector for overexpression of Rbfox1 in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox1 (Rbfox1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CAGGS-EGFP

Plasmid

#22212

Purpose

AAV zinc finger donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Aug 13. ():.

Insert

CAGGS-eGFP

Use

Zfn donor

Tags

Expression

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pattB-LSL-AAVR-F2A-spCas9

Plasmid

#202459

Purpose

The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.

Depositor

Jan Carette

Article

Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d

Insert

AAVR (AU040320 Mouse)

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

mCherry fusion

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-K-RAS V12

Plasmid

#31200

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

K-Ras (KRAS Human)

Use

Gateway donor vector

Tags

Expression

Mutation

G12V constitutively active

Promoter

Available since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
pCRISPRyl

Plasmid

#70007

Purpose

CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2015 Dec 29.

Inserts

Codon optimized Cas9 from S. pyogenes
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-hPhyB621-mCherry-HRasCT

Plasmid

#100282

Purpose

Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome B (1-621 aa) (PHYB Mustard Weed)

Use

Tags

mCherry fused with HRas C-terminus for plasma mem…

Expression

Mammalian

Mutation

Human codon optimized PhyB (1-621 aa)

Promoter

CAG promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAG426GPD-ccdB-YFP-yeastSMASh

Plasmid

#68855

Purpose

Expresses SMASh tag fused to YFP at C terminus in yeast

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

YFP-yeastSMASh

Use

Tags

SMASh tag

Expression

Yeast

Mutation

Promoter

GPD

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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