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Plasmid#79517PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTb427.10.7420
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceJune 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHL1948
Plasmid#53046PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM10:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterpConNoHind, pConNoHindM10, and pConNoHindM12Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1947
Plasmid#53045PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM8:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterpConNoHind, pConNoHindM12, and pConNoHindM8Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
PP-CDPK1
Plasmid#25280PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertcgd5_820:D42-E517 (cgd5_820 Cryptosporidium parvum)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationContains amino acids D42-E517.PromoterAvailable sinceFeb. 2, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV Retrograde)
Viral Prep#83896-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomato (physically separate, not a fusion protein)Available sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV Retrograde)
Viral Prep#83897-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomatoAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Easy-MISE toolkit
Plasmid Kit#1000000230PurposeThe Easy-Modular Integrative fuSion-ready Expression (Easy-MISE) toolkit is a complete vector set leading to fast engineering procedures for the improvement of heterologous production in S. cerevisiaeDepositorAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBASceI
Plasmid#26477PurposeI-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI siteDepositorInsertpCBASceI
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CT-sfGFP-HN-GG
Plasmid#215803PurposeCloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-CTH10STII-GG
Plasmid#215804PurposeCloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiFC-CrN173
Plasmid#22016DepositorInsertCrN173
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
LeGO-Cer2
Plasmid#27338DepositorInsertSFFV promoter, Cerulean
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
8 pCOLI_A_GST
Plasmid#160150PurposeN-term GST 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOpen-GBDpol (exo)
Plasmid#165572PurposeRobust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEBDepositorInsertPyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4
Plasmid#217971PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.DepositorInsert2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723
Plasmid#48015PurposePlease see depositor comments section.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
28 pCOLI_S_STREP
Plasmid#160262PurposeN-term 2xStrepII 3C cleavable Spec resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepIIExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
XLone-eGFP NLS (no drug resistant gene)
Plasmid#140032PurposeTunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSANG14-3F
Plasmid#39265DepositorTypeEmpty backboneUseTags6xHis, Alkaline phosphatase, pelB leader, and triā¦ExpressionBacterialMutationPromoterT7Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only