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Plasmid#67143PurposeGateway destination vector for cell-free expression with N-term 8xHis sfGFP tagDepositorTypeEmpty backboneUseCell-free expressionTagsHis and sfGFPExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only
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pSX2720
Plasmid#200764PurposeExpresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.DepositorInsertscFv-sfGFP
UseTagsExpressionWormMutationPromotercol-19Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPC034
Plasmid#171153PurposepGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassetteDepositorInsertJ3(106)- BBa_J23111-sfGFP
UseTagsExpressionBacterialMutationPromoterJ3(106)-BBa_J23111Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT040A
Plasmid#159401PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoterDepositorInsertpr.J23117/J23106-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterhybrid J23117-J23106 promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12
Plasmid#69942PurposepSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanRDepositorInsertTheoHHAzRaj12,cisRaj12,sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj11
Plasmid#69939PurposepSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanRDepositorInsertTheoHHAzRaj11,cisRaj11,sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKbreak12
Plasmid#69938PurposepSTC2,cisRaj12-sfGFP, KanRDepositorInsertCisRaj12-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12-o11
Plasmid#69941PurposepSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanRDepositorInsertTheoHHAzRaj12,cisRaj11,sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBFC1207
Plasmid#186694PurposeVcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right EndDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
UseTagsExpressionBacterialMutationPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNP1723
Plasmid#222727PurposesfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)DepositorTypeEmpty backboneUseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 Lysosomal-METRIQ
Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT sfGPF 150TAG
Plasmid#154766Purposeplasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG in GFP reporter, U25C in PylTPromoterEF1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD237-3a-2
Plasmid#90957PurposeExpresses ThsR and mCherry constitutively and sfGFP under the PphsA promoterDepositorInsertsThiosulfate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23100, Bba_J23105, and PphsAAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD239-1g-2
Plasmid#90959PurposeExpresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoterDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23100, Bba_J23109, and PttrB185-269Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-GFP (MoonTag-Nb-GFP)
Plasmid#128602PurposeExpresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and sfGFPExpressionMutationPromoterSFFVAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS423-GPD-wrmScarlet1-10-CYC1 terminator
Plasmid#158584PurposeExpresses wrmScarlet1-10 in S. cerevisiaeDepositorInsertwrmScarlet1-10
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD184
Plasmid#90953PurposeExpresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutivelyDepositorInsertsThiosulfate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23114, PltetO-1, and PphsAAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD233.7-3
Plasmid#90955PurposeExpresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutivelyDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23114, PltetO-1, and PttrB185-269Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAD-SPS6-Pman
Plasmid#133906PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBL7056
Plasmid#167225Purposeorthogonal T7 RNAP sfGFP reporterDepositorInsertpAKSIRV-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only