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Plasmid#50573PurposeExpresses FLAG-tagged mouse UBR2 in mammalian cells.DepositorInsertUBR2 (Ubr2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
opto-RhoGEF-mCherry_pcDNA3.1
Plasmid#164473PurposeExpresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.DepositorInsertopto-RhoGEF
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-BLMP-1-pcDNA3.1-
Plasmid#52512Purposeexpresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertBlmp-1 (blmp-1 Nematode)
UseTagsFLAG-HAExpressionMammalianMutationN75S mutation compared GenBank reference sequence…PromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/VChR1-EYFP
Plasmid#20955PurposeMammalian expression of VChR1 fused to EYFP, for neuronal excitationDepositorInsertvolvox channelrhodopsin-1
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma8-GFP2
Plasmid#140990PurposeEncodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma8-GFP2 (GNG8 Human)
UseTagsGFP2 and GSAG linkerExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα11-LgB97
Plasmid#134361PurposeNanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.DepositorInsertGα11-LgB97 (GNA11 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-StrepHA-SUMO2
Plasmid#66867PurposeMammalian expression of SUMO2 with a strep and an HA tagDepositorInsertSUMO2 (SUMO2 Human)
UseTagsHA and StrepExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GoldenGate-VP64
Plasmid#47389PurposeExpression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning systemDepositorTypeEmpty backboneUseTALENTagsFLAG, HA, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4-mammalian-recoded_mCherry_pcDNA3.1
Plasmid#114595PurposeEncodes mCherry-tagged BcLOV4 for expression in mammalian cellsDepositorInsertBcLOV4
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-V5 Myc
Plasmid#176049PurposeExpresses Myc tagged with His and V5 in Mammalian cellsDepositorInsertMyc
UseTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD40L-mgSrtA
Plasmid#125795Purposefor co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.DepositorInsertCD40L-mgSrtA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX
Plasmid#52421PurposeExpresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherryDepositorInsertFusion NLS-EGFP-P2A-Cherry-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα13-LgB106
Plasmid#134364PurposeNanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.DepositorInsertGα13-LgB106 (GNA13 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRoquin1_AE
Plasmid#148611PurposeMammalian Expression of HsRoquin1DepositorInsertHsRoquin1 (RC3H1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag HBx
Plasmid#42596DepositorInsertHepatitis B virus protein X
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_C-6His-Stx17
Plasmid#112259PurposeC-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)DepositorInsertsyntaxin 17 (Stx17 Mouse)
UseTags6His TagExpressionMammalianMutationPromoterT7Available sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR2(T/A)
Plasmid#161754PurposeNegative-control mutant for ExRai-AKAR2 biosensor.DepositorInsertExRai-AKAR2(T/A)
UseTagsExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-EGFP_AB
Plasmid#148304PurposeMammalian Expression vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag mHMGB2
Plasmid#31610DepositorInsertHMGB2 (Hmgb2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-Firefly
Plasmid#87066PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal Firefly luciferaseDepositorTypeEmpty backboneUseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only