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Addgene
Showing: 781 - 800 of 8038 results

  1. pcDNA3.1.D2-NP

    Plasmid
    #134366
    Purpose
    Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    D2-NP (DRD2 Human)
    Use
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    134366-plasmid-map-sequence-id-306391

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  3. pcDNA3.1-N-Luc

    Plasmid
    #185357
    Purpose
    For mammalian expression of N-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 2-416).
    Depositor
    Ayaz Najafov
    Insert
    Firefly Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    185357-plasmid-map-sequence-id-363972

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  4. pcDNA3.1-C-Luc

    Plasmid
    #185358
    Purpose
    For mammalian expression of C-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 398-550).
    Depositor
    Ayaz Najafov
    Insert
    Firefly Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    185358-plasmid-map-sequence-id-376433

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  5. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Use
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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  6. pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394

    Plasmid
    #128387
    Purpose
    Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc398-3xGGGGS-LGALS8 (LGALS8 Synthetic, Human)
    CALCOCO2-3xGGGGS-CLuc394 (CALCOCO2 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    CLuc394 and NLuc398
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and IRES
    Availability
    Academic Institutions and Nonprofits only
    128387-plasmid-map-sequence-id-261539

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  9. pGL3-alphaMHC 3300bp

    Plasmid
    #51934
    Purpose
    luciferase reporter for rat alpha-MHC promoter
    Depositor
    Jeffery Molkentin
    Insert
    alpha MHC (Myh6 Rat)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    -2930 to +410 promoter region
    Promoter
    alpha MHC
    Availability
    Academic Institutions and Nonprofits only

  10. pJKW 0633

    Plasmid
    #74122
    Purpose
    Photinus pyralis sulfotransferase ST2 - pHis8-4
    Depositor
    Jing-Ke Weng
    Article
    Fallon et al Biochemistry. 2016 May 26.
    Insert
    Photinus pyralis sulfotransferase 2
    Use
    Tags
    8x-His, TEV protease site (ENLYFQ)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    74122-plasmid-map-sequence-id-142571

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  11. RL-Lin28

    Plasmid
    #120520
    Purpose
    Renilla luciferase reporter
    Depositor
    Petr Svoboda
    Insert
    Renilla luciferase (Lin28a Mouse, Synthetic)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120520-plasmid-map-sequence-id-233816

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  12. RL-Elavl2

    Plasmid
    #120521
    Purpose
    Renilla luciferase reporter
    Depositor
    Petr Svoboda
    Insert
    Renilla luciferase (Elavl2 Mouse, Synthetic)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120521-plasmid-map-sequence-id-233989

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  13. pAP1-3

    Plasmid
    #71258
    Purpose
    luciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Insert
    3 copies of an AP-1 site from the human Stromelysin-1 gene (MMP3 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    GM-CSF minimal
    Availability
    Academic Institutions and Nonprofits only
    71258-plasmid-map-sequence-id-131888

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  20. pGL4.10-Rnd3 promoter [-1759 +248]

    Plasmid
    #86438
    Purpose
    Firefly luciferase reporter under control of the Rnd3 promoter
    Depositor
    Violaine Moreau
    Insert
    Rnd3 promoter (RND3 Human)
    Use
    Luciferase
    Tags
    Luciferase (under Rnd3 promoter control)
    Expression
    Mammalian
    Mutation
    promoter sequence derived from HepG2 cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    86438-plasmid-map-sequence-id-170466

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Showing: 781 - 800 of 8038 results