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Plasmid#85438PurposeLentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry reporter and constitutive tBFP expressionDepositorInsertS. typhi FliC
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry2_TagBFP
Plasmid#83031PurposeExpresses full length sfCherry2 and TagBFP fusion in mammalian cellsDepositorInsertsfCherry+full length_TagBFP
UseLentiviralTagsExpressionMammalianMutation3 mutations in sfCherry full length: E118Q, T128I…PromoterSFFVAvailable sinceJan. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_mTagBFP2_synCoTC
Plasmid#199474PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_mTagBFP2_synCoTC
Plasmid#199472PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
UseTagsExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
Plasmid#45579PurposeExpresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani K227M
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
UseTagsExpressionMammalianMutationA191GPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSA_1_mTagBFP2_synCoTC
Plasmid#199473PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
Plasmid#176311PurposeExpresses NLS-pMag-CreC60 with tagBFP markerDepositorInserttagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMammalianMutationPromoterhuman EF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExp_CAG:EBFP2
Plasmid#51790PurposeConstitutively expresses blue fluorescent protein, EBFP2DepositorInsertEBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
tTA_BFP
Plasmid#58854PurposeConstitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)DepositorInserttTA transcription factor fused to enhanced blue fluorescent protein
UseTagsBFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_BFP2_WPRE
Plasmid#91787PurposeConstitutive expression of mTagBFP2 using PGK promoter in a lentiviral backbone.DepositorInsertmTagBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB18cmBFP1A5
Plasmid#14035DepositorInsertBFP1A5
UseYeast/bacteria optimized codonsTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
sgRNA.SFFV.tBFP
Plasmid#169940PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tBFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP
Plasmid#45574PurposeExpresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backboneDepositorInsertco I-Sce I
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
XClone_Cas9_KRAB_T2A_BFP
Plasmid#141208PurposeTunable and temporal expression control of dCas9DepositorInsertdCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBFP2
Plasmid#165831PurposeEBFP2blue fluorescent protein CDSDepositorInsertEBFP2
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_EBFP_Donor
Plasmid#187455PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N EBFP donor (ATP1A1 Human)
UseTagsExpressionMammalianMutationPromoterhPGK1Available sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45564PurposeExpresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequenceDepositorInsertsd14 eGFP donor
I-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationdeleted n' 15 amino acidsPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-ER-5
Plasmid#55240PurposeLocalization: Endoplasmic Reticulum, Excitation: 383, Emission: 448DepositorInsertER (CALR Human)
UseTagsEBFP2ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits