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Plasmid#119067PurposeDonor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertNLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
Plasmid#119065PurposeDonor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertHA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc CDC4 WT*
Plasmid#16652DepositorInsertCDC4 (FBXW7 Human)
UseTagsmycExpressionMammalianMutation*Note that this insert is only WT relative to the…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5C-FLAG
Plasmid#72196PurposeExpresses full-length Unc5c with a C-terminal FLAG tagDepositorInsertUnc5c (Unc5c Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ai32
Plasmid#34880PurposeCre-dependent expression of ChR2(H134R)-EYFP, for optogenetic excitationDepositorInsertFloxed ChR2(H134R)-EYFP
UseMouse TargetingTagsEYFPExpressionMutationH134R in ChR2PromoterCAGAvailable sinceMay 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-CUL1-3'UTR
Plasmid#97156PurposeFor dual luciferase report assayDepositorInsertCUL1 3'UTR (CUL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Zscan4 promoter
Plasmid#69249PurposeLuciferase reporter for Dux4 activation of Zscan4 transcriptionDepositorInsertZscan4 (ZSCAN4 Human)
UseLuciferaseTagsExpressionMutationThe two single nucleotide mismatches found betwee…PromoterZscan4Available sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_sfCherry2(11)_Clathrin light chain
Plasmid#83032PurposeExpresses sfCherry2(11) tagged clathrin light chain in mammalian cellsDepositorInsertsfCherry2(11)_Clathrin light chain (CLTA Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 508)
Plasmid#195030PurposeGAL4-Creb5 fusion construct (full length, 508aa)DepositorInsertGAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5A-FLAG
Plasmid#72194PurposeExpresses full-length Unc5a with a C-terminal FLAG tagDepositorInsertUnc5a (Unc5a Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-aa1-149
Plasmid#73410PurposeExpresses HA tagged aa1-149 of AKT1DepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationExpresses aa 1-149 only of AKT1PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-aa120-433
Plasmid#73411PurposeExpresses HA tagged aa120-433 AKT1DepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationExpresses aa 120-433 of AKT1PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
p7SL-neo-TET
Plasmid#51285Purposeexpresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassetteDepositorInsert7SL RNA (RN7SL2 Human)
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoter7SL upstream pollII enhancerAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-STIM1
Plasmid#89817PurposeExpress HUMAN STIM1DepositorInsertSTIM1 (STIM1 Human)
UseRetroviralTagsNO TAGExpressionMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIMVAu1
Plasmid#98301PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.DepositorInsertHMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
scaffold-linker-U6
Plasmid#89639Purposeconstruction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-aa1-408
Plasmid#73412PurposeExpresses HA tagged aa1-408 of AKT1DepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationExpresses aa 1-408 of AKT1PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-NL-DHFR-SpCas9
Plasmid#124522PurposeExpresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cellsDepositorInsertNL-DHFR-SpCas9
UseAAVTagsDestabilized domain of E.coli dihydrofolate reduc…ExpressionMammalianMutationPromoterCbhAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5D-FLAG
Plasmid#72197PurposeExpresses full-length Unc5d with a C-terminal FLAG tagDepositorInsertUnc5d (Unc5d Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S197A
Plasmid#131522PurposeExpresses MKP-3 with S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Serine 197 to AlaninePromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only