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Plasmid#115145PurposeSafe harbor site 231 knock-in vector with RFP-HygromycinR expression cassetteDepositorInsertRFP
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC150-KEIMA-RAMP4
Plasmid#201929PurposePiggyBac Vector expressing KEIMA-RAMP4DepositorInsertRAMP4 (SERP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC150-KEIMA-REEP5
Plasmid#201928PurposePiggyBac Vector expressing KEIMA-REEP5DepositorInsertREEP5 (REEP5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNIL BSD-mApple
Plasmid#182313PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mAppleUseTagsT2A-mycNLS-mAppleExpressionMammalianMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_FLAG-Mx PylRS YA
Plasmid#140012PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
UseTagsFLAGExpressionMammalianMutationY126APromoterEF1Available sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast
Plasmid#205565PurposeExpress human NEDD4L (delta C2 isoform)DepositorInsertNEDD4L (NEDD4L Human)
UsePiggybacTagsExpressionMammalianMutationdelta C2 isoformPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB[vasa-Cas9 PUb-ZsGreen]
Plasmid#169012PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster vasaAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-REVERBA-mScarlet-NLS-PEST
Plasmid#190658PurposeFluorescent reporter for circadian rhythmsDepositorInsertReverba-mScarlet (Nr1d1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6c
Plasmid#117223PurposeExpresses gRNA in Aedes aegyptiDepositorInsertHR U6c promoter-gRNA
UseSynthetic BiologyTagsExpressionInsectMutationPromoterU6Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6b
Plasmid#117222PurposeExpresses gRNA in Aedes aegyptiDepositorInsertHR U6b promoter-gRNA
UseSynthetic BiologyTagsExpressionInsectMutationPromoterU6Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-Zim3-MeCP2
Plasmid#220143Purposedoxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repressionDepositorInsertdCas9-Zim3-MeCP2
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTre-3GAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast
Plasmid#205551PurposeExpress EPN24-MCP-I-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP-I
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKH151_PB_flag-LC3B_wt_IRES-BFP
Plasmid#214976PurposeFlag tagged LC3BDepositorInsertFLAG-LC3B (MAP1LC3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo
Plasmid#102885PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-CXXC(24)
Plasmid#124398PurposeFull-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)
UseTagsFlagExpressionMammalianMutationCXXC zinc finger deletion (aa 585-624)PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-BGHpA
Plasmid#92161PurposeFor lncRNA overexpression (in trans)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAEL010097-Cas9
Plasmid#100707PurposeThe promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL010097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL010097 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast
Plasmid#205552PurposeExpress EPN24-MCP-p6-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP-p6
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9GFP-IRES-Neo
Plasmid#102907PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.DepositorInsertDDdCas9GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only