pAS_4xMx PylT_FLAG-Mx PylRS YA
(Plasmid
#140012)
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PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140012 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAS
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMx1201 PylRS YA
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Alt name4xh7SK-Mx1201 PylT cassette
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SpeciesMethanomethylophilus alvus Mx1201
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Insert Size (bp)885
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MutationY126A
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Entrez GeneMMALV_RS05520 (a.k.a. MMALV_RS05520, MMALV_11280)
- Promoter EF1
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAS_4xMx PylT_FLAG-Mx PylRS YA was a gift from Simon Elsaesser (Addgene plasmid # 140012 ; http://n2t.net/addgene:140012 ; RRID:Addgene_140012) -
For your References section:
Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells. Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ. ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571. 10.1021/acschembio.8b00571 PubMed 30260624