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Plasmid#225236PurposeAAV expression of iPASnFR-L from hSyn promoterDepositorInsertiPASnFR-L
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-RF1-His6
Plasmid#224372PurposeExpress RF1 protein for his-tagged purificationDepositorInsertRF1 (prfA E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG
Plasmid#225242PurposeMammalian expression of iPASnFR-L in the cytosolDepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJYDC1
Plasmid#162458PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the NterminusDepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHA tag, HDEL sequence, eUnaG2 fluorescent protein…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 VDAC1-Linker-GFP
Plasmid#211734PurposeExpresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 VDAC1-TC
Plasmid#211733PurposeExpresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsTetracysteineExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV5cap
Plasmid#198015PurposeFor high-level expression of the AAV5 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV5 cap
UseAAVTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG
Plasmid#225246PurposeMammalian expression of iPASnFR-L in the nucleusDepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-cox8-iPASnFR-L-mScarlet3-3xFLAG
Plasmid#225240PurposeMammalian expression of iPASnFR-L in mitochondriaDepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mScarletI3-6DG5-MAPPER
Plasmid#222421PurposeTo express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)DepositorInsertMAPPER
UseTagsmScarletI3-6DG5ExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msTMEM115-3C-3xHA-P2A-eGFP
Plasmid#214997PurposeStably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.DepositorInsertTMEM115 (Tmem115 Mouse)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterUBCAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG
Plasmid#225241PurposeMammalian expression of iPASnFR-H in the cytosolDepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L
Plasmid#196136PurposeSB vector for the mammalian expression of GADD34-K3LDepositorInsertGADD34 Δ1-240 - P2A - K3L (PPP1R15A Human, vaccinia virus)
UseTagsP2AExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-PLIN3
Plasmid#222420PurposeTo express a lipid droplet "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertPLIN3 (PLIN3 Human)
UseTagsFRB-mCherryExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-VPS35
Plasmid#166220PurposeGateway entry vector for N. furzeri VPS35DepositorArticleInsertVPS35
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-2A-mTagBFP2
Plasmid#207746PurposeExpression of the Cre recombinase linked to a mTagBFP2 fluorescent markerDepositorArticleInsertCre-PGK-mTagBFP2
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF1α-Cre-2A-miRFP670nano3
Plasmid#207747PurposeExpression of the Cre recombinase linked to a miRFP670nano3 fluorescent markerDepositorArticleInsertCre-PGK-miRFP670nano3
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTwist GFP-Linker-VDAC1
Plasmid#211735PurposeExpresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT32
Plasmid#204297PurposepJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -