Addgene

We narrowed to 35,036 results for: LEA

Showing: 7621 - 7640 of 35036 results

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pMIG-AVITEV- hNFATc1/aA

Plasmid

#74057

Purpose

retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc1, isoform alpha-A (NFATC1 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Mutation

mutation G1157A [R235Q] and the silent mutation C…

Promoter

pMSCV-LTRs

Availability

Academic Institutions and Nonprofits only

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pOTTC588 - pAAV c-fos Nuc-iRFP

Plasmid

#59132

Purpose

An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Infrared Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

mFos

Availability

Academic Institutions and Nonprofits only

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pOTTC589 - pAAV c-fos Nuc-eYFP

Plasmid

#59133

Purpose

An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Enhanced Yellow Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

mFos

Availability

Academic Institutions and Nonprofits only

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pCDNA3 IRF7(5’UTR)-FF

Plasmid

#85490

Purpose

Firefly luciferase under the control of IRF7 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of IRF7 (Irf7 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

Plasmid

#79889

Purpose

Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

Plasmid

#70667

Purpose

Human mutant of E2F1 lacking amino acids 193-359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 193 to 359 (E2F1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

lacks amino acids 193-359

Promoter

cmv

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

Plasmid

#70669

Purpose

Human mutant of E2F1 lacking amino acids 284 to 359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 284 to 359 (E2F1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

lacks amin acids 284 to 359

Promoter

cmv

Availability

Academic Institutions and Nonprofits only
pICE-HA-CtIP-siR-S664A-S679A-S745A

Plasmid

#82031

Purpose

Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Patrick Calsou, Sébastien Britton

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

CtIP (RBBP8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Non phosphorylatable mutations on 3 of the main A…

Promoter

CMV-tet

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin S14C

Plasmid

#60614

Purpose

Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

S14C

Promoter

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin G13R

Plasmid

#60615

Purpose

Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

G13R

Promoter

Availability

Academic Institutions and Nonprofits only

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pHAGE GFP-Ago2 S387A

Plasmid

#60654

Purpose

GFP-Ago2 S387A mutant lentiviral vector

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

S387A

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pHAGE GFP-Ago2 S387E

Plasmid

#60655

Purpose

GFP-Ago2 S387E mutant lentiviral vector

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

S387E

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pGEX 4T-1 GST-Ago2 S387A

Plasmid

#60656

Purpose

Expresses GST-Ago2 S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

S387A

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon hChR2(H134R)-EYFP

Plasmid

#55644

Purpose

Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

nEF

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

Plasmid

#55645

Purpose

Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde, AAV5, AAV8, and AAV9

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

hSyn

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

Plasmid

#55646

Purpose

Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

hSyn

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Foff hChR2(H134R)-EYFP

Plasmid

#55647

Purpose

Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Short Ef1a

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

Plasmid

#55648

Purpose

Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre off/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

hSyn

Availability

Academic Institutions and Nonprofits only

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pAAV-hnEF Coff/Fon hChR2(H134R)-EYFP

Plasmid

#55649

Purpose

Cre-off/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre off/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Short Ef1

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha270-1374S300/302A

Plasmid

#62533

Purpose

N-terminal aa1-269 of Drosha is deleted and serines 300 &302 are mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

truncated human Drosha mutant (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269 and changed serines 300…

Promoter

Availability

Academic Institutions and Nonprofits only