pACS131
(Plasmid
#204596)
-
PurposeHistone H3.1K27M-V5
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepLENTI6.3-TOPO/TA
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7692
- Total vector size (bp) 8101
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namehistone H3.1 K27M
-
SpeciesH. sapiens (human)
-
Insert Size (bp)410
-
GenBank ID8350
-
Entrez GeneH3C1 (a.k.a. H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A)
- Promoter CMV
-
Tag
/ Fusion Protein
- human histone H3.1-V5 (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer V5-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pACS131 was a gift from Tom Misteli (Addgene plasmid # 204596 ; http://n2t.net/addgene:204596 ; RRID:Addgene_204596) -
For your References section:
Identification of epigenetic modulators as determinants of nuclear size and shape. Schibler AC, Jevtic P, Pegoraro G, Levy DL, Misteli T. Elife. 2023 May 23;12:e80653. doi: 10.7554/eLife.80653. 10.7554/eLife.80653 PubMed 37219077
