Addgene

We narrowed to 45,022 results for: sta

Showing: 7561 - 7580 of 45022 results

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pTT3-ecdCD80-ST3-His

Plasmid

#210665

Purpose

Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)

Use

Tags

Histag and Spytag003

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO shLYCHOS/LYCHOS WT-FLAG

Plasmid

#209315

Purpose

Lentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V1-rCD20-BSD

Plasmid

#209752

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-rCD20-BSD

Plasmid

#209753

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-rCD20-BSD

Plasmid

#209754

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-EGFL7-COMP5AP-AviTag-9xHis

Plasmid

#157266

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EGFL7 (EGFL7 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGK-Ldb3-mKate2

Plasmid

#128766

Purpose

Fluorescent mKate reporter for Ldb3

Depositor

Deepak Srivastava

Article

Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

Insert

Ldb3 promoter (Ldb3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ldb3

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-GUCY2C-G549C

Plasmid

#116407

Purpose

Lentiviral expression of GUCY2C G549C

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GUCY2C (GUCY2C Human)

Use

Lentiviral

Tags

Expression

Mutation

G549C

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) TSPAN9

Plasmid

#107502

Purpose

Expresses TSPAN9, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

TSPAN9 (TSPAN9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFUSEss-chimeric-IgM-mouse/human_M18

Plasmid

#91738

Purpose

Expression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence.

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

Insert

immunoglobulin heavy constant mu (Ighm Mouse)

Use

Tags

Expression

Mammalian

Mutation

Residues 203-239 (EU numbering) were exchanged to…

Promoter

hEF1-HTLV prom

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mM-Asp212Ser_M18

Plasmid

#91739

Purpose

Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ser (EU numbering) - N-glycosylated Asn209

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

Insert

immunoglobulin heavy constant mu (Ighm Mouse)

Use

Tags

Expression

Mammalian

Mutation

Asp212 changed to Ser (EU numbering)

Promoter

hEF1-HTLV prom

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133K

Plasmid

#83447

Purpose

Mammalian expression of SOD1E133K

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E133K

Promoter

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSOD1E133insTT-AcGFP1

Plasmid

#83441

Purpose

Expresses SOD1E133insTT in mammalian cells.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

E133insTT

Promoter

CMV

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSOD1E133Δ-AcGFP1

Plasmid

#83419

Purpose

Expresses SOD1E133Δ in mammalian cells.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

E133 deletion

Promoter

CMV

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-LOC442075

Plasmid

#23372

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC442075 (LOC442075 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Nsp3 Mac1 - C2 (UCSF)

Plasmid

#169209

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Article

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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His6-TEV-CRBN-midi

Plasmid

#215330

Purpose

CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

Depositor

Alessio Ciulli

Article

Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

Insert

CRBN (CRBN Human)

Use

Tags

6xHis and TEV

Expression

Bacterial

Mutation

contains amino acids 41-187 followed by a Gly-Ser…

Promoter

lacI

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV-NAT-FLAG-CIP2A FL (sg2R)

Plasmid

#222624

Purpose

Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF-1-alpha promoter

Available since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3-human OCT4 DE-SV40-Luc

Plasmid

#52414

Purpose

Luciferase reporter plasmid for DE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Distal element of human OCT4 promoter (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) EPB41L1

Plasmid

#107508

Purpose

Expressing EPB41L1, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

EPB41L1 (EPB41L1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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