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We narrowed to 11,160 results for: cel.2

Showing: 7521 - 7540 of 11160 results

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pLVX-IRES-Gαq*-BERKY3

Plasmid

#158425

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-Brn2

Plasmid

#32925

Purpose

Retroviral expression of mouse Brn2

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Brn2 (Pou3f2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
PM PKA Reporter

Plasmid

#104551

Purpose

Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Lyn-mTurquoise2-VASP 148 - 164

Use

Tags

mTurquoise 2, HA tag

Expression

Mammalian

Mutation

Promoter

EF1 alpha

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαi*-BERKY1

Plasmid

#158090

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-KB1753-myc

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαi*-BERKY2

Plasmid

#158197

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-2xYFP-KB1753-myc

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gα13(EE)-YFP

Plasmid

#171374

Purpose

Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Ga13(EE)-YFP

Use

Tags

Glu-Glu

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Gαi*-BERKY3

Plasmid

#158424

Purpose

BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Use

Lentiviral

Tags

myc

Expression

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5-ERRbeta2

Plasmid

#52186

Purpose

Expresses human ERRbeta2 splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Use

Tags

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+ Lifeact-mCherry

Plasmid

#185408

Purpose

Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

lifeact

Use

In vitro synthesis of mrna

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225062

Purpose

Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Use

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225063

Purpose

Expression of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Use

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSG5-ERRbeta-delta10

Plasmid

#52187

Purpose

Expresses human ERRbeta-delta10 splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Use

Tags

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
KRP85

Plasmid

#24448

Depositor

Ron Vale

Article

Pierce et al Biochemistry. 1999 Apr 27. 38(17):5412-2

Insert

Kinesin II (KRP85 Sea urchin)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
KRP95

Plasmid

#24447

Depositor

Ron Vale

Article

Pierce et al Biochemistry. 1999 Apr 27. 38(17):5412-2

Insert

Kinesin II (KRP95 Strongylocentrotus purpuratus)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
Lourido Toxoplasma CRISPR Library V1

Pooled Library

#80636

Purpose

Plasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes.

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-MLG-EGFP-LST2

Plasmid

#220785

Purpose

Expresses N-terminal EGFP-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al PNAS 2024

Insert

LST2 (ZFYVE28 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRB30

Plasmid

#165480

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Use

Tags

TEV-FLAG-10x His

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-MLG-HMF-LST2

Plasmid

#220786

Purpose

Expresses N-terminal His10-Myc-FLAG-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al PNAS 2024

Insert

LST2 (ZFYVE28 Human)

Use

Tags

10xHis-Myc-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAceBAC2-MLG-HMF-LST2

Plasmid

#220787

Purpose

Expresses N-terminal His10-Myc-FLAG-LST2 in insect cells

Depositor

Timm Maier

Article

Battaglioni et al PNAS 2024

Insert

LST2 (ZFYVE28 Human)

Use

Tags

10xHis-Myc-FLAG

Expression

Insect

Mutation

Promoter

p10

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRB33

Plasmid

#165482

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Use

Tags

10xHis-FLAG-TEV

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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