Addgene

We narrowed to 14,714 results for: yeast

Showing: 7441 - 7460 of 14714 results

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pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)

Plasmid

#14846

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)

Use

Tags

ECFP

Expression

Yeast

Mutation

TATA-A1T6. TATA box mutated from TATATAAG to AATA…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 PHO5(UASm1)pr VYFP (EB#1549)

Plasmid

#14841

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter UASm1 (PHO5 Budding Yeast)

Use

Tags

VYFP

Expression

Yeast

Mutation

UASm1 (AAGCTT was substituted for CACGTT). Unindu…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)

Plasmid

#14844

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter TATA-C2 (PHO5 Budding Yeast)

Use

Tags

ECFP

Expression

Yeast

Mutation

TATA-C2. TATA box mutated from TATATAAG to TCTA…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pT-36

Plasmid

#16252

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

Insert

CDC3 (CDC3 Budding Yeast)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evolution 2024

Insert

SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Q443P

Plasmid

#216401

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Q443P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Glutamine 443 to Proline, and deleted ami…

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pXOOY

Plasmid

#196449

Purpose

A dual vector for expression of membrane proteins in S. cerevisiae and X. laevis

Depositor

Per Amstrup Pedersen

Article

Vold et al PLoS One. 2023 Feb 21;18(2):e0281868. do

Type

Empty backbone

Use

Expression in xenopus laevis

Tags

Expression

Yeast

Mutation

Promoter

yeast CYC-GAL and T7 promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN100

Plasmid

#205073

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 100 amino acids

Promoter

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN380

Plasmid

#205076

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 380 amino acids

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN296

Plasmid

#205075

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 296 amino acids

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pYP-ptef1-GFP

Plasmid

#199990

Purpose

Yeast/E coli shuttle vector expressing GFP from tef1 promoter

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Edc3

Plasmid

#201413

Purpose

Bacterial protein expression of Edc3

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Pat1

Plasmid

#201414

Purpose

Bacterial protein expression of Pat1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp2

Plasmid

#201411

Purpose

Bacterial protein expression of Dcp2

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp1

Plasmid

#201410

Purpose

Bacterial protein expression of Dcp1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRS306-PTDH3-SUMO7-6SIM

Plasmid

#190250

Purpose

Expression SUMO7-SIM6 in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

7SUMO-6SIM

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRS304-PTDH3-HEXAMER

Plasmid

#190251

Purpose

Expression hexamer component in yeast

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

hexamer

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Hygro-Bem1-mCherry-AID

Plasmid

#173925

Purpose

Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

Depositor

Liedwij Laan

Article

Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

Inserts

Partial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence

Use

Tags

mCherry- AID

Expression

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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LDB34

Plasmid

#185431

Purpose

Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)

Depositor

Laura Davis, Ken Belanger

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Use

Tags

Expression

Yeast

Mutation

Truncation at amino acid 1041 from the C-term of …

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Laura Davis, Ken Belanger

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Use

Tags

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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