Addgene

We narrowed to 13,597 results for: pET

Showing: 7441 - 7460 of 13597 results

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pZac2.1-GfaABC1D-GAT3-HA-mCherry

Plasmid

#117722

Purpose

Expresses a GABA transporter GAT-3 (Slc6a11)

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Slc6a11 (Slc6a11 Mouse)

Use

AAV

Tags

HA and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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BPK2301

Plasmid

#65778

Purpose

Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

St1Cas9 gRNA backbone, without spacer sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pVRc20_992

Plasmid

#49739

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS20_992

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pSNRW-Z5-Nluc

Plasmid

#174487

Purpose

Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

Depositor

Richard C. Willson

Article

Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

Insert

Z5-Nluc

Use

Tags

6x Histidine tag (N terminal on insert)

Expression

Bacterial

Mutation

Promoter

T7lac promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 rictor

Plasmid

#21341

Depositor

David Sabatini

Article

Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

Insert

rictor shRNA 1 (Rictor Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6f

Plasmid

#112000

Purpose

For enriched expression of GCaMP6f in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV1

Insert

axon-GCaMP6f

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2PHR

Plasmid

#28244

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

cry2 (CRY2 Mustard Weed)

Use

Tags

Gal4BD

Expression

Yeast

Mutation

Promoter

Available since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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MMLV-RT for Split-PE (pEH45)

Plasmid

#190105

Purpose

Expresses MMLV-RT (full-length, from PE2) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT

Use

Tags

bpNLS

Expression

Mammalian

Mutation

RT pentamutant from PE2 (D200N, T306K, W313F, T33…

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee-NLS

Plasmid

#140900

Purpose

mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-NLS

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee-NES

Plasmid

#140901

Purpose

mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-NES

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLVTHM-shN

Plasmid

#83046

Purpose

Control shRNA pLVTHM-shN

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA Neg Control

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE-H1

Available since

Oct. 24, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-Amp_CRISPIE donor B2

Plasmid

#172843

Purpose

CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mRuby3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pVRb22_up1147

Plasmid

#49716

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pecf22_up1147

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb03_up1198

Plasmid

#49706

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pecf03_up1198

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pVRc03_1198

Plasmid

#49730

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS03_1198

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
MSP977

Plasmid

#65774

Purpose

Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E mutation in Cas9

Promoter

CMV & T7

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAM4937 (pCV0049)

Plasmid

#120082

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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gfp-Arkadia/RNF111

Plasmid

#112228

Purpose

Expresses GFP-tagged Arkadia in mammalian cells

Depositor

Vasso Episkopou

Article

Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

Insert

(Arkadia)Rnf111 (Rnf111 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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