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Plasmid#61575PurposeTarget a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 geneDepositorInsertSnap25-LSL-2A-GFP
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB09-TRE-beta-globin-miR-124-EF1a-GFP
Plasmid#117318PurposeOverexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intronDepositorInserthsa-miR-124-1 (MIR124-1 Human)
UseTransposon-mediated integrationTagsGFPExpressionMammalianMutationPromoterTet-inducible promoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA
Plasmid#65453PurposeCan be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinasesDepositorHas ServiceAAV1InsertEGFP
UseAAVTagsExpressionMutationPromotertetOAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-bREACHes-EYFP
Plasmid#137147PurposeIntersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT CreDepositorInsertCoff/Fon-bREACHes-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP
Plasmid#117319PurposeOverexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intronDepositorInserthsa-miR-CTRL
UseTransposon-mediated integrationTagsGFPExpressionMammalianMutationmiR-124 deletedPromoterTet-inducible promoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA(TARDBP)
Plasmid#109323PurposeAAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBPDepositorInsertmCherry-KASH (TARDBP Human)
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hRSK3(RPS6KA2)_K91A_K444A
Plasmid#180385Purposelentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activityDepositorInsertRPS6KA2_K91A/K444A (RPS6KA2 Human)
UseLentiviralTagsExpressionMammalianMutationK91A/K444APromoterCMVAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-eGFP-NLS-RNF168 delta RING
Plasmid#133983Purposemammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
UseTagseGFPExpressionMammalianMutationdeletion of RING; siRNA resistant sequence: GAGGA…PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV superYFP-AURKA K162M-mTurq2
Plasmid#157773PurposeExpression of AuroraA kinase-dead biosensor under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2 and superYFPExpressionMammalianMutationAURKA K162M is a kinase-dead version of AURKAPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-PHD2-181-426
Plasmid#223551PurposeHis-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purificationDepositorInsertPHD2 (EGLN1 Human)
UseTags6x HisExpressionBacterialMutationDeleted amino acids 1-180.PromotertrcAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-FMR1-dCGG0
Plasmid#222965PurposeTo create 0CGG FMR1 cell lineDepositorInsertFMR1 homologous template no CGG repeats (FMR1 Human)
UseCRISPRTagsExpressionMutationCGG repeats deleted from 5' UTRPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-FLAG-msMMS22L
Plasmid#221015Purposemammalian expression vector of Flag tagged mouse MMS22LDepositorInsertMMS22L (Mms22l Mouse)
UseTagsFlagExpressionMammalianMutationnaturally siResistant to siMMS22L (human) aagacuu…PromoterCMVAvailable sinceSept. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/TO-eGFP-msMMS22L
Plasmid#221014Purposemammalian expression vector of eGFP tagged mouse MMS22LDepositorInsertMMS22L (Mms22l Mouse)
UseTagseGFPExpressionMammalianMutationnaturally siResistant to siMMS22L (human) aagacuu…PromoterCMVAvailable sinceSept. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-Flag-RECQL5
Plasmid#222620PurposeMammalian expression vector of flag-tagged RECQL5DepositorInsertRECQL5 (RECQL5 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-Flag-BRIP1
Plasmid#222619PurposeMammalian expression vector of flag-tagged BRIP1DepositorInsertBRIP1 (BRIP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRX-IP-muGFP-hATG2A ΔWIR
Plasmid#215507PurposeExpresses GFP tagged human ATG2A with the mutation in WIPI interacting region.DepositorInsertAutophagy related 2A (ATG2A Human)
UseRetroviralTagsmuGFPExpressionMammalianMutationP656R (natural variant with no functional relevan…PromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-muGFP-hATG2A mLIR
Plasmid#215506PurposeExpresses GFP tagged human ATG2A with the mutation in LC3 interacting region.DepositorInsertAutophagy related 2A (ATG2A Human)
UseRetroviralTagsmuGFPExpressionMammalianMutationP656R (natural variant with no functional relevan…PromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-U6-sgHTT1-7sk-sgCas9
Plasmid#190900PurposeAAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAsDepositorInsertsgRNA (HTT spCas9, Human)
UseAAV and CRISPRTagsExpressionMutationPromoterU6 and 7skAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVss-U6-sgHTT51-7sk-Cas9
Plasmid#190901PurposeAAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNADepositorInsertsgRNA (Htt Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterU6 and 7skAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1
Plasmid#190902PurposeAAV vector expressing thew GFP reporter gene and human sgHTTDepositorInsertGFP and sgHTT (HTT Human)
UseAAV and CRISPRTagsExpressionMutationPromoterEFS and U6Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only