Addgene

We narrowed to 29,354 results for: uros

Showing: 7401 - 7420 of 29354 results

Results per page:

Sort results by:

Cav2.1 EIVA (E1707A) pcDNA3

Plasmid

#206125

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Use

Tags

Expression

Mammalian

Mutation

E1707A

Promoter

CMV

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.1 HA EIA (E320A) pcDNA3

Plasmid

#206124

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Use

Tags

Expression

Mammalian

Mutation

E320A

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

Plasmid

#65458

Purpose

Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

tTA2

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-Con/Foff 2.0-GCaMP6F

Plasmid

#137123

Purpose

Intersectional viral expression of GCaMP6F in cells expressing Cre AND NOT Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-GCaMP6F

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

Removed RSET tag

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2AK2 gRNA (BRDN0001146342)

Plasmid

#75637

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK2 (EIF2AK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP

Plasmid

#137149

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing Cre AND NOT Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-Arch3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP

Plasmid

#137139

Purpose

Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing both Cre AND Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-ChR2(ET/TC)-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

E123T, T159C

Promoter

nEF

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-mCherry-Tandem-PSAM4-GlyR

Plasmid

#208361

Purpose

Expression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.

Depositor

David Bennett

Article

Perez-Sanchez et al Sci Transl Med. 2023 Oct 4;15(716):eadh3

Insert

mCherry-TANDEM-PSAM4-GlyR

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416-Gal-PR50

Plasmid

#84901

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Use

Tags

FLAG and Myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
hSyn-GtACR2-ChRmine-tandem-eYFP

Plasmid

#192580

Purpose

AAV-vector for bidirectional optogenetic manipulation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

somBiPOLES

Use

AAV

Tags

EYFP- soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Pvalb-2A-Cre targeting vector

Plasmid

#61570

Purpose

Target the Cre recombinase gene to the stop codon of the mouse Pvalb gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb exon 4 - 2A - Cre

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP

Plasmid

#137146

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Cre AND NOT Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Con/Foff 2.0-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Pvalb-2A-Flpo Flp-in replacement vector

Plasmid

#61572

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Flpo

Use

Recombinase-mediated cassette exchange using flp …

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP

Plasmid

#137150

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-Arch3.3-p2a-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-RPS14(WT)-Myc

Plasmid

#122235

Purpose

expresses RPS14 protein with a Myc tag in mammalian cells (puro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

vector's LTR

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2AK2 gRNA (BRDN0001148668)

Plasmid

#75639

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK2 (EIF2AK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_TBK1_WT

Plasmid

#82285

Purpose

Gateway Donor vector containing TBK1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TBK1 (TBK1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

N388D; K570Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Con/Foff 2.0-ChR2-mCherry

Plasmid

#137143

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

H134R

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416-Gal-PA50

Plasmid

#84902

Purpose

induce expression of dipeptide repeat PA in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PA50 (C9orf72 Synthetic)

Use

Tags

FLAG and Myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Con/Fon-bREACHes-EYFP

Plasmid

#137145

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Con/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge