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Plasmid#207461PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertTdTc302 (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS1-TEV-HAT
Plasmid#202557PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
UseTagsHistidine-Affinity-tag (HAT) and TEV cleavable si…ExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS2-TEV-Twin-Strep
Plasmid#202529PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cellDepositorInsertSGMS2 (SGMS2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TGA)PromoterCAG and chicken β-actin promoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFDPS #2
Plasmid#198760Purposeconditional knockdown of FDPSDepositorInsertshFDPS #2 (FDPS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFDPS #1
Plasmid#198759Purposeconditional knockdown of FDPSDepositorInsertshFDPS #1 (FDPS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D
Plasmid#198765Purposeexpression of hOCT4 mutantsDepositorInserthOCT4 236D (POU5F1 Human)
UseTagsFLAGExpressionMammalianMutationS236DPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chr1q_Cassette-Proximal_gRNA
Plasmid#195127PurposegRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controlsDepositorInsertChr1q gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax
Plasmid#190154PurposeExpresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertVamp3 (Vamp2 Rat)
UseAAVTagsP2AT2A-EGFP-caaxExpressionMammalianMutationTruncation that includes only rat Vamp3 AA #1-81PromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5-STABLE2-neo-RvCAHS3
Plasmid#192472PurposeStable expression of RvCAHS3 (fly codon optimized) in Drosophila cellsDepositorInsertCAHS3
UseTagsT2A-EGFP-T2A-neoRExpressionInsectMutationPromoterAc5 promoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS97splitRBP_S2-Enz
Plasmid#183768PurposeExpresses SHIP2-Enzyme domain flanked by RBPDepositorInsertSHIP2 (INPPL1 Human)
UseTags6HIS, RBP (C-terminal) HRV3C cleavage site, and R…ExpressionBacterialMutationincludes aa K414 to aa V872 onlyPromoterT7lacAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA2
Plasmid#160210PurposeKnock Down MTLSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA3
Plasmid#160212PurposeKnock Down MYDSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA1
Plasmid#160208PurposeKnock Down MQWSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(+7A)-IDR-YFP-NLS
Plasmid#145278PurposeMammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLSDepositorInsertHOXD13(+7A)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXA13(+7A)-IDR-mCherry
Plasmid#145285PurposeBacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion ProteinDepositorInsertHOXA13(+7A)-IDR (HOXA13 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXA13(WT)-IDR-mCherry
Plasmid#145284PurposeBacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion ProteinDepositorInsertHOXA13(WT)-IDR (HOXA13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13(+7A)-IDR-mCherry
Plasmid#145281PurposeBacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion ProteinDepositorInsertHOXD13(+7A)-IDR (HOXD13 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXD11-Adel-IDR
Plasmid#145241PurposeExpresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDRDepositorInsertHOXD11-Adel-IDR (HOXD11 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXA13+7A-IDR
Plasmid#145239PurposeExpresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDRDepositorInsertHOXA13+7A-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HEY2wt-IDR
Plasmid#145235PurposeExpresses fusion of GAL4 DNA-binding domain and HEY2wt IDRDepositorInsertHEY2wt-IDR (HEY2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only