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Plasmid#52262PurposeBacterial expression of human 6His-tagged SUMO2 and SUMO-E1DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only
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pAAV-EF1a-N-CretrcintG
Plasmid#69570PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - N-CretrcintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-4 dCARD
Plasmid#208785PurposeInducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2DepositorInsertCry2 mCherry Caspase4 dCARD (CASP4 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationCARD domain is removedPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-C-CreintG
Plasmid#69571PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - C-CreintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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SiC-V2-Cas9G7
Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-JNKKTRClover
Plasmid#59139PurposeORF encoding JNK KTR mClover flanked by Gateway sequencesDepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2_SEPT9 i4
Plasmid#71617Purposemammalian expression of human SEPT9 i4 with a GFP fusionDepositorInsertSeptin 9 (SEPTIN9 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Mitbow
Plasmid#158994PurposeMulticolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertMitbow
UseTagsThe default expressed EBFP2 is fused to H2B; oth…ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB402-omega
Plasmid#74797PurposeGateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter2xCaMV35S enhancer and omegaAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-Sumo-TEV human OAS1
Plasmid#130895PurposeHuman oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobosDepositorInsertoligoadenylate synthase 1 (OAS1 Human)
UseTagsStrep_SumoExpressionInsectMutationPromoterphAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-LATS2-KR
Plasmid#33100DepositorInsertlats2 (Lats2 Mouse)
UseTagsHAExpressionMammalianMutationK655R, Kinase DeadPromoterCMVAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-mCherry-Syk
Plasmid#50045PurposeExpression of mCherry-Syk in mammalian cellsDepositorInsertSpleen tyrosine kinase (Syk) (Syk Mouse)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-p38beta
Plasmid#86833Purposemammalian expression of GFP-p38betaDepositorInsertp38beta (MAPK11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-PKI nls
Plasmid#118301PurposeExpression of PKIaDepositorInsertPKI nls (aa 2-36)
UseTagsFlag and GFPExpressionMammalianMutationinserting 2xnlsPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-DIO-tTA
Plasmid#99121PurposeAn AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTADepositorHas ServiceAAV1InsertDIO-tTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterihSyn1Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-FAK-HA-V744G
Plasmid#35040DepositorInsertfocal adhesion kinase (Ptk2 Mouse)
UseTags2x HA and mCherryExpressionMammalianMutationGFP-FAK-V744G was generated from GFP-FAK using th…PromoterAvailable sinceMarch 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5 DN-Fyn
Plasmid#16033DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationDominant negative: K299MPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-NTwinStrep
Plasmid#45940Purposeuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneUseTagsHIS-6-tag, PelB export signal, and Twin-Strep-tag…ExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -