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Showing: 7201 - 7220 of 27176 results

  1. pDual-eGFP(H66)

    Plasmid
    #63558
    Purpose
    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(H66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. GFP-I48 ELP

    Plasmid
    #67857
    Purpose
    GFP Fluorescent Elastin-like Polypeptide(VPGIG) with 48 repeats containing Isoleucine guest residues
    Depositor
    Andrew MacKay
    Insert
    GFP fused to ELP (VPGIG) repeated 48 times
    Use
    Tags
    GFP fused to N-terminal of ELP
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    67857-plasmid-map-sequence-id-117815

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  4. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid
    #85820
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc Dam_f_GFPoNLS
    Use
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A.
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85820-plasmid-map-sequence-id-170474

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  12. pUASTLOTattB_mCherry-CD8-vhhGFP

    Plasmid
    #163930
    Purpose
    Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer
    Depositor
    Markus Affolter
    Article
    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Insert
    Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163930-plasmid-map-sequence-id-323460

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  13. GFP-CRY2-P14P5K

    Plasmid
    #79569
    Purpose
    expression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c
    Depositor
    Olof Idevall-Hagren
    Article
    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26
    Insert
    Pip5k1c (Pip5k1c Mouse)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    R445E and R446E; truncation of amino acids 636-66…
    Promoter
    CMV
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. GFP-itis pBE-t

    Plasmid
    #195342
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V
    Depositor
    Alexis Komor
    Article
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA ctctacgcgggtcttgtagt
    Use
    CRISPR
    Tags
    Expression
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195342-plasmid-map-sequence-id-383223

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Showing: 7201 - 7220 of 27176 results