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Plasmid#63558PurposeExpression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(H66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-I48 ELP
Plasmid#67857PurposeGFP Fluorescent Elastin-like Polypeptide(VPGIG) with 48 repeats containing Isoleucine guest residuesDepositorInsertGFP fused to ELP (VPGIG) repeated 48 times
UseTagsGFP fused to N-terminal of ELPExpressionMammalianMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET30 pEGFP-N2 (815)
Plasmid#62011Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET30 (MOSPD3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
NET5L pEGFP-N2 (1155)
Plasmid#62035Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET5L (TMEM201 Human)
UseTagsEGFPExpressionMammalianMutationlong formPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
SIVURA MCP-GFPenvy
Plasmid#189940PurposeSingle-integration vector for integration of MCP-GFPEnvy at the URA3 locus in Saccharomyces cerevisiaeDepositorInsertMCP-GFPEnvy
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-N-P-M-EGFP-SynPhRFP-L
Plasmid#52483PurposeExpresses EGFP and Synaptophysin-TagRFP-TDepositorInsertsEGFP
Synaptophysin-TagRFP-T
UseRabies virusTagsTagRFP-TExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5A-Rigor
Plasmid#172202PurposeExpresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cellsDepositorInsertKIF5A (Kif5a Mouse)
UseTagseGFPExpressionMammalianMutationChanged Glycine 235 to AlaninePromoterCMVAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-BIRC3-LU
Plasmid#160700PurposeExpresses GFP-BIRC3 with the long 3'UTR in mammalian cellsDepositorInsertGFP-BIRC3 (BIRC3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNL-EGFP/TRETightdU3
Plasmid#18660DepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
prNFL-Q333P-EGFP
Plasmid#132602PurposeExpresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E Q333P mutationDepositorInsertNefl (Nefl Rat)
UseTagsEGFPExpressionMammalianMutation998A>C, 999G>C; Gln333ProPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry-CD8-vhhGFP
Plasmid#163930PurposeExpression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-CRY2-P14P5K
Plasmid#79569Purposeexpression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1cDepositorInsertPip5k1c (Pip5k1c Mouse)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationR445E and R446E; truncation of amino acids 636-66…PromoterCMVAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PML peptide
Plasmid#175246PurposeBacterial expression and purification, low affinity SUMOylation substrateDepositorInsertPML (PML Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S181E
Plasmid#105312PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS181EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S181A
Plasmid#105307PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS181APromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-mCh-GFPi
Plasmid#21905DepositorInsertGFPi
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-itis pSel
Plasmid#195344PurposeSelection plasmid expressing non-fluorescent EGFP A111VDepositorInsertEGFP
UseCRISPRTagsExpressionMutationA111VPromoterJ23119Available sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-itis pBE-t
Plasmid#195342PurposeBase editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110VDepositorInsertsecTadA(8e)-SpCas9-NG
gRNA ctctacgcgggtcttgtagt
UseCRISPRTagsExpressionMutationSpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…PromoterLac and ProCAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3G
Plasmid#169315PurposeDoxycycline-inducible expression of miR-125b-2 with dTomato as reporter fluorescent proteinDepositorInsertmiR-125b-2 (MIR125B2 Human)
UseLentiviralTagsdTomatoExpressionMutationPromoterTREAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only