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Plasmid#39486DepositorInsertARAP3(R307,8A) (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationR307,8A = PH domain mutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3(C504A)
Plasmid#39485DepositorInsertARAP3(C504A) (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationC504A = Arf GAP domain mutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-HOIL-1 helix-RBR (233–510)
Plasmid#193859PurposeExpression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coliDepositorInsertHOIL-1 (RBCK1 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationResidues 233–510, codon optimised for expression …PromoterT7Available sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 3xFlag
Plasmid#110063PurposeFGFR2 expression in mammalian cells (JCOPCO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 F276C 3xFlag
Plasmid#110064PurposeFGFR2 F276C expression in mammalian cells (JCOPO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Phenylalanine 276 to Cysteine (F276C)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 K41E 3xFlag
Plasmid#110105Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Lysine 41 to Glutamic acid (K41E)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_S100A9_KRT4
Plasmid#205916PurposeExpress mEGFP-tagged fusion protein, S100A9_KRT4 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP8G-ccdB
Plasmid#83884PurposeDestination vector for integration at HIS3, expression from the GPD promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP6G-ccdB
Plasmid#83880PurposeDestination vector for integration at yeast URA3 locus, expression from the GPD promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP4G-cccdB
Plasmid#83876PurposeDestination vector for integration at yeast Trp locus, expression from the GPD promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP5G-ccdB
Plasmid#83879PurposeDestination vector for integration at yeast Leu locus, expression from the GPD promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOpen-T4 PNK
Plasmid#165520PurposeThe most commonly used polynucleotide kinase.DepositorInsertT4 PNK
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 3, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Tthlig
Plasmid#165569PurposeTaq DNA LigaseDepositorInsertTth DNA Ligase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-T4gene43
Plasmid#165524PurposePolymerase that replicates viral genomic DNA.DepositorInsertT4 DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Pwopol
Plasmid#165562PurposeDNA polymerase; additionally exhibits 3' to 5' exonuclease activity.DepositorInsertPwo DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-ECOpolA
Plasmid#165532PurposeDNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.DepositorInsertE. coli DNA Polymerase I
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-EcoRIR
Plasmid#165504PurposeType II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.DepositorInsertEcoRI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-EcoRV
Plasmid#165548PurposeType II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.DepositorInsertEcoRV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-PstIR
Plasmid#165526PurposeRestriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.DepositorInsertPstI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-T7gene1
Plasmid#165505PurposeHighly processive DNA-dependent RNA polymerase derived from T7 bacteriophageDepositorInsertT7 RNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits