pOPINB-HOIL-1 helix-RBR (233–510)
(Plasmid #193859)

• Purpose: Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli
• Depositing Lab: Bernhard Lechtenberg
• Publication: Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi: 10.1038/s41467-023-35871-z.

Backbone

• Vector backbone: pOPINB
• Backbone size w/o insert (bp): 5642
• Total vector size (bp): 6147
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HOIL-1
• Alt name: Heme-oxidized IRP2 ubiquitin ligase 1
• Alt name: RBCK1 C20orf18, HOIL-1, HOIL1, PBMEI, PGBM1, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4
• Alt name: RanBP-type and C3HC4-type zinc finger-containing protein 1
• Species: H. sapiens (human)
• Mutation: Residues 233–510, codon optimised for expression in E. coli
• GenBank ID: NM_031229
• Entrez Gene: RBCK1 (a.k.a. C20orf18, HOIL-1, HOIL1, PBMEI, PGBM1, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6x-His tag (N terminal on backbone)
  • 3C protease cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Resource Information

• Supplemental Documents:
  • BCL1317 pOPINB-HOIL-1Lopt1_233-510.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Helix-RBR construct suitable for in vitro activity assays and crystallisation