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Plasmid#90482PurposeTargeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicumDepositorInsertgroup II intron and ItrA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tom20-CIB-stop
Plasmid#117243PurposeLight activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switchDepositorInsertCIBN
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-I-N-CAAX
Plasmid#41686PurposeExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activationDepositorInsertDronpa145N-IntersectinDH-Dronpa145N-CAAX
UseTagsCAAX boxExpressionMammalianMutationK145N on DronpaPromoterCMVAvailable sinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBJ5 BRG1 DN
Plasmid#17874DepositorInsertBrg DN (SMARCA4 Human)
UseTagsExpressionMammalianMutationdominant negativePromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-PAT1
Plasmid#88856Purposemyc-TAG overexpressionDepositorInsertSLC36A1 (SLC36A1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shLuc
Plasmid#83092PurposeLentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)DepositorInsertshLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
VAChT-pHuji
Plasmid#105319PurposeVAChT tagged with pH-sensitive red fluorescent protein pHujiDepositorInsertVAChT (Slc18a3 Rat)
UseTagspHujiExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4
Plasmid#80601PurposeExpresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Plk4(Sak) D154A (Nigg HR26)
Plasmid#41164DepositorInsertPlk4 D154A (PLK4 Human)
UseTags3xMyc and PreScission SiteExpressionMammalianMutationD154APromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
YFP-ICP0FxE-C3
Plasmid#134561PurposeExpresses YFP-ICP0 fusion protein with a RING finger deletion (FXE)DepositorInsertICP0
UseTagsYFPExpressionMammalianMutationRING finger deletion (FXE)PromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCS2 hSmad1-EVE
Plasmid#22993DepositorInsertSmad1 (SMAD1 Human)
UseTagsFLAGExpressionMammalianMutationS463E, S465EPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S
Plasmid#149638Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, emptyDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL70008
Plasmid#50336DepositorInsertpFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113342PurposeExpresses dCas9-p300 and MS2-p65-HSF1 fusion proteinsDepositorInsertdCas9-p300core-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Eef1a-1955/-1>nls::Cas9::nls
Plasmid#59987PurposeEef1a (EF1alpha) promoter driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Eef1a (EF1alpha) -1955/-1Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH87633
Plasmid#50271DepositorInsertpromoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA
Plasmid#112921PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant markerDepositorInsertEF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMrfp
Plasmid#59773PurposeDerived from pCM62; ampR, tetR, PBAD - rfpDepositorInsertrfp
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113344PurposeExpresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteinsDepositorInsertVP64-dCas9-p300core-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only