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Plasmid#68436PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-EEA-guide1-PGK-Puro
Plasmid#102904PurposeEBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-guide1-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
OA-1055J
Plasmid#200251PurposepBac-U6-gRNA(Intersex)-3xp3-tdTomatoDepositorInsert2 gRNAs targeting Intersex gene
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pM378
Plasmid#173174PurposeExpresses C9-1 gRNA-12xMBS from hU6 promoterDepositorInsertC9-1 targeting gRNA-12xMBS
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
sg2.0 (PP7) Locus#2
Plasmid#101155PurposegRNA with two PCP binding sitesDepositorInsertLocus #2(tandem repeat)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-UGCG-KO
Plasmid#80010PurposegRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.DepositorInsertUGCG (UGCG Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
sg2.02 MUC4.1
Plasmid#101152PurposegRNA with two MCP binding sitesDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromotermU6Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKC123
Plasmid#134240Purposeexpresses gRNA targeting CENP A 3'DepositorInsertCENP A 3' CRISPR construct
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Non-targeting CRISPRi plasmid
Plasmid#154891PurposeHuman non-targeting CRISPRi gRNADepositorInsertNontargeting gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-TdT-guide1-PGK-Puro
Plasmid#102903PurposeEBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.DepositorInsertTdT-guide1-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBW1997_pExpr-hU6-CRISPR-DECODER
Plasmid#87549PurposeExpresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinasesDepositorInsertgRNAs targeting NGN2, MIAT, ACTC1, TTN
UseCRISPR, Cre/Lox, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC393
Plasmid#91221Purposeprotoplast vector expressing gRNA24 targeting tomato ANT1 (with TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC440
Plasmid#91225Purposeprotoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression)DepositorInsertgRNA targeting barley MLO
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC436
Plasmid#91222Purposeprotoplast vector expressing gRNA38 targeting barley MLO (with TREX2 expression)DepositorInsertgRNA targeting barley MLO
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGK58
Plasmid#228118PurposeExpress node 814 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 814, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAGK65
Plasmid#228123PurposeExpress node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1550, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK59
Plasmid#228119PurposeExpress node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1429, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK57
Plasmid#228117PurposeExpress node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 482, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only