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Plasmid#138148PurposeExpresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInsertCasRx
UseLentiviralTagsNESExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_MOKV_G
Plasmid#207294PurposeMammalian expression of Mokola virus G proteinDepositorInsertMOKV_G (G Mokola virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-BDFP1.6
Plasmid#197199PurposeExpresses the protein of BDFP1.6 in mammalian cellsDepositorInsertBDFP1.6
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)
Plasmid#114082PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
UseTags2x SV40 NLS and SV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimi…PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-EGFP-ADRP
Plasmid#87157PurposeFluorescent protein localized to lipid droplets (LDs)DepositorInsertADRP (PLIN2 Human)
UseTagsEGFPExpressionMammalianMutationPromotermCherry-R TTGGTCACCTTCAGCTTGGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
AiP1038-pAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpA
Plasmid#163475PurposeDirect-expressing FlpO AAV Virus. Alias: AiP1038-pAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpAInsertFlpO
UseAAVTagsExpressionMutationPromoterBeta Globin minimal promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST
Plasmid#164235Purposelentiviral plasmid for GFP-TREX1 delta N (aa236-314)DepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationdeletion of amino acids 1-234PromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-N
Plasmid#108078PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorInsertCCDB (ccdB(letD) )
UseTagsStrepIII-HA-BirA*ExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GFP TEV LIC cloning vector (1GFP)
Plasmid#29663DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHis6-GFP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51083-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato (#51083). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato plasmid DNA. Cre-dependent GCaMP6f calcium sensor and physically separate nuclear tdTomato, under control of the EF1a promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pN1-RnKIF5C(1-560,Δ6)-Halo-Flag
Plasmid#188072PurposeMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.DepositorInsertrat KIF5C (Kif5c Rat)
UseTagsFlag and HaloExpressionMammalianMutationaa1-560, deletion of aa2-6PromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-6XHis-human SUMO 2
Plasmid#133771Purposeexpress 6Xhis- SUMO 2 proteinDepositorInsertSUMO 2 (SUMO2 Human)
UseTags6XHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIK
Plasmid#126554PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 Flag BAF155 b
Plasmid#1954DepositorInsertBAF155 (SMARCC1 Human)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
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CN2249-rAAV-eHGT_451m-minBglobin-SYFP2-WPRE3-BGHpA
Plasmid#164452PurposeDirect-expressing SYFP2 AAV VirusInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBetaGlobinAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
AbVec1.1-IGKC
Plasmid#80796PurposeExpression of immunoglobulin light chains in mammalian cells, human kappa constant regionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP (AAV8)
Viral Prep#137140-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP (#137140). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Flp) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Cre-dependent)Available sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
hHS1-M7A
Plasmid#159170PurposeHuman codon-optimized cytosolic labile heme reporterDepositorInserthHS1-M7A
UseTagsExpressionMammalianMutationMutated Met 7 of the cyt b562 module of hHS1 to A…PromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only