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Plasmid#128137PurposeThe cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1201
Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.7)-BE4
Plasmid#108381PurposeMammalian expression vector for xCas9(3.7)-BE4DepositorInsertxCas9(3.7)-BE4
UseTagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIZ-Flag6His-BmAgo3
Plasmid#50559PurposeExpresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cellsDepositorInsertBmAgo3
UseTags6xHIS and FlagExpressionInsectMutationPromoterOpIE2Available sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Neo-tdTomato
Plasmid#47975PurposeTrasgenically expresses tandem tdtomato fluorescent protein in T. cruziDepositorInserttdTomato
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSD080A
Plasmid#218138PurposeLentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.DepositorInsertSD40-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-HA-human CMYC S62D
Plasmid#203437PurposeMammalian expression of MYCDepositorInsertMYC (MYC Human)
UseTags2 x HAExpressionMammalianMutationS62DPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP4
Plasmid#42313DepositorInsertVAMP 4 (VAMP4 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-HA-human CMYC S62A
Plasmid#203436PurposeMammalian expression of MYCDepositorInsertMYC (MYC Human)
UseTags2 x HAExpressionMammalianMutationS62APromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCbs Flag CMYC (bHLH/LZ)
Plasmid#203424PurposeMammalian expression of MYCDepositorInsertMYC (MYC Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Dvl2-GFP
Plasmid#109148PurposeStable Dvl2 expressionDepositorInsertDvl2 (DVL2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterSV40Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:DsRed:Tnos (GB0361)
Plasmid#68220PurposeTranscriptional unit for DsRed plant expression driven by the 35S promoterDepositorInsertDsRed
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP3
Plasmid#42310DepositorInsertVAMP 3 (VAMP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9
Plasmid#84245PurposeExpresses direct fusion KRAB-Sp dCas9DepositorInsertsKRAB-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, KRAB, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Ls-AChBP
Plasmid#63126PurposeExpression of Lymnaea stagnalis AChBPDepositorInsertAChBP
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET14b – E. coli Exonuclease I
Plasmid#104552Purposebacterial expression of Exonuclease IDepositorInsertExonuclease I (sbcB E.coli)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRH623
Plasmid#171633Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-non-targeting
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA (MSW#111)
Plasmid#208066Purposeexpression of human Replication Protein A in E. coliDepositorUseTagsExpressionBacterialMutationRPA32 has a point mutation Thr88(ACC) -> Ile(A…PromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TO-DEST
Plasmid#106348PurposeDestination vector for gateway cloning. Developed for Flp-In T-REx system.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-LK03
Plasmid#206512PurposeAAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoterDepositorInsertLK03 capsid
UseAAVTagsExpressionMutationPromoterp5Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only