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Plasmid#179988PurposeMammalian expression vector for RaTG13 ORF3a, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 ORF3a
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCS2+Cas9-mSA
Plasmid#103882PurposeCas9 mSA for in vitro transcription for gene editing in Mouse EmbryosDepositorInsertCAS9-MSA
UseCRISPRTagsMSA (monomeric streptavidinExpressionMutationnonePromoterSP6Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)
Plasmid#188375PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)
Plasmid#107941PurposeMammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutionsDepositorInserthuman codon optimized enAsCas12a (E174R/S542R/K548R)
UseTags3x HA and NLS (nucleoplasmin)ExpressionMammalianMutationE174R, S542R and K548RPromoterCAGAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)
Plasmid#112022PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-betaDepositorInsertNYESO alpha/beta into TRBC1 HDRDT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-Golgi eGFP
Plasmid#79809PurposeTo express and target GFP into the Golgi apparatus using lentivirusesDepositorInsertGolgi eGFP (B4GALT1 Human)
UseLentiviralTagsFusion of the first 62 amino acids of the human b…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
B2396_BTB-EGFP
Plasmid#215099PurposeExpresses BCL6 BTB(1-129)-EGFP in mammalian cellsDepositorInsertBTB-EGFP (BCL6 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ST-SBP-mCherry
Plasmid#65265Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
wtTDP43tdTOMATOHA
Plasmid#28205DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsHA tag and tdtomatoExpressionMammalianMutationPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJFRC19-13XLexAop2-IVS-myr::GFP
Plasmid#26224DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-VCPIP1
Plasmid#22592DepositorInsertVCPIP1 (VCPIP1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceDec. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-DRGFP
Plasmid#113193PurposeDR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.DepositorInsertDR-GFP
UseDonor construct with t2a-hygromycin for gene trap…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMVMagFRET-1
Plasmid#50742PurposeExpresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.DepositorInsertgene encoding for MagFRET-1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO p16 Hygro (w184-1)
Plasmid#22263DepositorInsertcyclin-dependent kinase inhibitor 2A (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJV62
Plasmid#26910DepositorInsertChe9c gene 61
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV Retrograde)
Viral Prep#162377-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
EMTB-mCherry
Plasmid#26742DepositorInsertensconsin (MAP7 Human)
UseProbe for microtubulesTags2x mCherry and MycExpressionMammalianMutationMicrotubule binding-domain of ensconsin (amino ac…PromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-enCas12a-2xNLS
Plasmid#209021PurposeLentiviral vector expressing human codon-optimized enCas12a with 2xNLSDepositorInsertenCas12a-2xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationE174R/S542R/K548RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG030 (TerY-P)
Plasmid#157908PurposeExpresses the TerY-P defense systemDepositorInsertTerY-P triad (vWA + kinase + phosphatase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma2-GFP2
Plasmid#166774PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma2-GFP2 (GNG2 Human, Synthetic)
UseTagsExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only