Skip to main content
Addgene
Showing: 681 - 700 of 4925 results

  1. pcDNA3.1-GGamma4-GFP2

    Plasmid
    #166776
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma4-GFP2 (GNG4 Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166776-plasmid-map-sequence-id-330999

    Enlarge

  5. pcDNA3.1(-)-flag-UBR2

    Plasmid
    #50573
    Purpose
    Expresses FLAG-tagged mouse UBR2 in mammalian cells.
    Depositor
    Alexander Varshavsky
    Insert
    UBR2 (Ubr2 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pcDNA3.1.Gα11-LgB97

    Plasmid
    #134361
    Purpose
    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα11-LgB97 (GNA11 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134361-plasmid-map-sequence-id-306399

    Enlarge

  17. pcDNA3.1(+)Gαi2-91-NLuc

    Plasmid
    #207149
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAI2 (GNAI2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)Q
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207149-plasmid-map-sequence-id-410273

    Enlarge

  20. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid
    #52421
    Purpose
    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry
    Depositor
    Bertrand Collet
    Insert
    Fusion NLS-EGFP-P2A-Cherry-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    52421-plasmid-map-sequence-id-81252

    Enlarge

Showing: 681 - 700 of 4925 results