Showing: 6961 - 6980 of 14114 results
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Plasmid#84738PurposeSimultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cellsDepositorInsertsUseTagsnuclear localisation signals from the coding sequ…ExpressionPlantMutationPromoterpFMV/p35SAvailabilityAcademic Institutions and Nonprofits only
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GFP-ING2(pHD)
Plasmid#21589DepositorInsertGFP-ING2(pHD) (Ing2 Mouse)
UseTagsEGFPExpressionMammalianMutationcontains Ing2 plant homeodomain (PHD) amino acids…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-FGFR1-Fv-Fvls-E
Plasmid#15285DepositorInsertFGFR1 kinase, FKBP12v36 (Fgfr1 Mouse)
UseTagsHA epitope and Myristoylation-targeting domain c-…ExpressionMammalianMutationFGFR1 cytoplasmic domain FKBP12 F36VPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-FGFR2-Fv-Fvls-E
Plasmid#15286DepositorInsertFGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)
UseTagsMyristoylation-targeting domain c-Src (14aa) and …ExpressionMammalianMutationFGFR2 cytoplasmic domain FKBP12 F36VPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-FGFR3-Fv-Fvls-E
Plasmid#15287DepositorInsertFGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)
UseTagsMyristoylation-targeting domain c-Src (14aa) and …ExpressionMammalianMutationFGFR3 cytoplasmic domain only FKBP12 F36VPromoterAvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS300E/S302D
Plasmid#62531PurposeSerine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acidDepositorInserthuman Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 300 to glutamic acid and serine 3…PromoterAvailabilityAcademic Institutions and Nonprofits only -
cryS96
Plasmid#67519PurposeαB-crystallin minipeptide fused to ELP (S96).DepositorInsertmini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)
UseTagselastin-like polypeptide (ELP) S96ExpressionBacterialMutationmini-αB-crystallin contains residues 73–92 of aB-…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
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pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro
Plasmid#132773PurposeExpression of human LMNA.DepositorInsertLamin A (LMNA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)
Plasmid#110761PurposeBacterial expression of proISG15DepositorInsertISG15 (ISG15 Human)
UseTagsintein/chitin binding domainExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N3::hTET3
Plasmid#136364PurposeExpresses epitope-tagged human TET3 WT in mammalian cellsDepositorInsertTET3 (TET3 Human)
UseTags2xStrep-tagII, FLAG, and HAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P66-ss
Plasmid#137045PurposeE. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66949.1 (p66 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P66: lacks the P66 signal peptidePromoterT7lacAvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-BB0238-ss
Plasmid#137046PurposeE. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66635.2 (BB_0238 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0238: lacks the BB0238 signal peptidePromoterT7lacAvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-RevA-ss
Plasmid#137064PurposeE. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAF07416.1 (revA Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationlacks the RevA signal peptide and 5 residues (CKA…PromoterT7lacAvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P13-ss
Plasmid#137065PurposeE. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66426.1 (BB_0034 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P13: lacks the P13 signal peptidePromoterT7lacAvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-HtrA-ss
Plasmid#137066PurposeE. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66500.2 (BB_0104 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature HtrA: lacks the HtrA signal peptidePromoterT7lacAvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-DipA-ss
Plasmid#137068PurposeE. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature DipA: lacks the DipA signal peptidePromoterT7lacAvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn G51D
Plasmid#105747PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein G51D (SNCA Human)
UseTagsExpressionBacterialMutationG51DPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3
Plasmid#105802PurposeConcomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-mRuby3; ORF2: TEV-mClover3ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
mTet1 flox donor
Plasmid#117147PurposeMaking Tet1 conditional knock outDepositorArticleInsertTet1 (Tet1 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 6961 - 6980 of 14114 results