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Plasmid#172727PurposeEncodes Part 1a of HexaPro-D614G spike to be used in the Spike Display systemDepositorInsertSARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
UseSynthetic BiologyTagsExpressionMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pU6-ccdB-boxB-tBE-V5-mA3
Plasmid#171693PurposeExpresses tBE-V5-mA3 in mammalian cellsDepositorInsertccdB-sgRNA scaffold-boxB-tBE-V5-mA3
UseCRISPRTagsExpressionMammalianMutationPromoterU6 and EF1aAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
HL13
Bacterial Strain#37976DepositorBacterial ResistanceChloramphenicolAvailable sinceJuly 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLX304-GW-DCK*-IRES-GFP
Plasmid#176291PurposeGateway vector for use in generating POI-DCK* fusion reporterDepositorInsertMutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
UseLentiviralTagsV5ExpressionMammalianMutationS74E R104M D133APromoterCMVAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-GW
Plasmid#60323PurposeThis is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).DepositorTypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX304-DCK*-GW-IRES-GFP
Plasmid#176289PurposeGateway vector for use in generating DCK*-POI fusion reporterDepositorInsertMutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
UseLentiviralTagsV5ExpressionMammalianMutationS74E R104M D133APromoterCMVAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)
Plasmid#169184PurposeBaculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cellsDepositorInsertnsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsHis6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)
Plasmid#169164PurposeBaculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cellsDepositorInsertnsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
UseTags6His-3xFlag (nsp10 C-terminus)ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)
Plasmid#185447PurposeCoexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
HL444
Bacterial Strain#69772PurposeMC4100 + agn43 gene deleted + ΔdamDepositorBacterial ResistanceChloramphenicol and kanamycinAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
UQ5853
Bacterial Strain#37159DepositorBacterial ResistanceChloramphenicolAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL372
Bacterial Strain#69771PurposeMC4100 + agn43 gene deleted + ΔOxyRDepositorBacterial ResistanceChloramphenicol and kanamycinAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
HL24
Bacterial Strain#37978DepositorBacterial ResistanceChloramphenicolAvailable sinceJuly 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 6845
Bacterial Strain#69785PurposeMC4100 + agn43 coding sequence replaced by T7RNAP and camR + Δdam + ΔSgrSDepositorBacterial ResistanceChloramphenicolAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
HL 6846
Bacterial Strain#69786PurposeMC4100 + agn43 coding sequence replaced by T7RNAP and camR + ΔOxyR + ΔSgrSDepositorBacterial ResistanceChloramphenicolAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
HL23
Bacterial Strain#37977DepositorBacterial ResistanceChloramphenicolAvailable sinceJuly 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL168
Bacterial Strain#37979DepositorBacterial ResistanceChloramphenicolAvailable sinceJuly 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
EcNR2.xseA-
Bacterial Strain#40802DepositorBacterial ResistanceChloramphenicol and ampicillinAvailable sinceOct. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 6692
Bacterial Strain#69776PurposeMG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + ΔOxyRDepositorBacterial ResistanceChloramphenicolAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
HL 6693
Bacterial Strain#69777PurposeMG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + ΔdamDepositorBacterial ResistanceChloramphenicolAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only