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Plasmid#50716Purpose5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.DepositorInsertsplit EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAHA
Plasmid#12517DepositorTypeEmpty backboneUseTagsDouble HA tagExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAJM.657
Plasmid#108525PurposeCymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.DepositorInsertCymRAM + PCymRC-YFP
UseTagsExpressionBacterialMutationCymR: S110G A171VPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21
Plasmid#105519PurposeT7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIV-H2B-eGFP
Plasmid#91776PurposeExpresses H2B protein tagged with eGFPDepositorInsertHuman Histone H2B / eGFP (H2BC11 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-HA-hTAF6
Plasmid#17988DepositorInsertTAF6 (TAF6 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hc-MYC
Plasmid#17220PurposeRetroviral expression of human cMYCDepositorInsertc-MYC (MYC Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (PX165)
Plasmid#48137PurposeHuman codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)DepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianMutationPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ140
Plasmid#99895PurposeEmpty vector with attL5-attL2 recombination sites for Gateway CloningDepositorTypeEmpty backboneUseCRISPR and TALENTagsExpressionMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-FLAG-CHK1
Plasmid#86875PurposeTo express CHK1 with a FLAG tag at N-terminus.DepositorInsertCHEK1 (CHEK1 Human)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJM.771
Plasmid#108534PurposeNahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.DepositorInsertNahRAM + PSalTTC-YFP
UseTagsExpressionBacterialMutationNahR: I25V P72H V118A R248CPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
TfR-pHuji
Plasmid#61505PurposeHuman transferrin receptor tagged with pH sensitive red fluorescent protein pHujiDepositorInsertTransferrin receptor (TFRC Human)
UseTagspHujiExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AKAP-mtagBFP2-UBC6
Plasmid#105007PurposeMammalian Expression of mtagBFP2DepositorInsertmtagBFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
anxA1-GFP
Plasmid#107194PurposeExpresses porcine annexin A1-GFP fusion protein for live cell imagingDepositorInsertannexin A1
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-bJunVN155(I152L)
Plasmid#27098DepositorInsertbJunVN155(I152L) (Jun and Aequorea Victoria, Rat)
UseTagsMycExpressionMammalianMutationI152L in Venus(1-154)PromoterAvailable sinceJan. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112922PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
PSD95-TS:YFP
Plasmid#42225DepositorInsertPSD95
UseTagsTS:YFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
ClopHensorN
Plasmid#50758PurposeMammalian expression vector expressing the ClopHensorN chloride and proton sensorDepositorInsertClopHensorN
UseTagsStrep-IIExpressionMammalianMutationE2GFP is EGFP-T203YPromoterCAGAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only