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Plasmid#91019PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pSTAP-seq_fly-zfh1
Plasmid#86381PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster zfh1 enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-VASP-5
Plasmid#55098PurposeLocalization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertVASP
UseTagsmCherry-miniSOGExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Peroxisomes-2
Plasmid#54847PurposeLocalization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528DepositorTypeEmpty backboneUseTagsPA-mCherry-miniSOG and Peroximal Targeting Signal…ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N1 cyclin B1 L45A (1324)
Plasmid#61851Purposemammalian expression of cyclin B1 L45 A mutant fused to VenusDepositorInsertcyclin B1 (CCNB1 Human)
UseTagsVenusExpressionMammalianMutationL45APromoterCMVAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-Rab5a-7
Plasmid#55094PurposeLocalization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertRab5a
UseTagsmCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 1
Plasmid#99316PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM549
Plasmid#112531PurposepZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpRDepositorInsertsHrtR
ChuA
UseTagsExpressionBacterialMutationPromoterJ23107 and ProDAvailable sinceFeb. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 hBad(1-101)
Plasmid#35568DepositorInsertbad (BAD Human)
UseTagsFlagExpressionBacterialMutationresidues 1-101PromoterAvailable sinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTC303
Plasmid#91210Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE76
Plasmid#80578Purposerecipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptIIDepositorInsertspnos:nptII-tmos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-dead_3
Plasmid#91167PurposeCas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:barDepositorInsertAtCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG484
Plasmid#91194PurposeWDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)DepositorInsertnTaCas9_H840A+gUbi8+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3306
Plasmid#91102PurposeModule C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterFMV 34SAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-4T-1-Shp2 SH2s
Plasmid#67575PurposeExpresses GST-tagged Shp2 SH2 domains in bacteriaDepositorInsertSrc homology 2 domain-containing phosphatase 2 SH2s
UseTagsGSTExpressionBacterialMutationDeleted amino acids 219-597PromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-2PH-PLCd
Plasmid#71283PurposeMultisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-deltaDepositorInsert2PH-PLC delta (Human)
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only