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Plasmid#49385PurposeExpresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cellsDepositorInsertConnexin43 - EGFP - APEX2
UseTagsExpressionMammalianMutationK14D, W41F, E112K, A134P on soybean APX; improved…PromoterCMVAvailable sinceNov. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pG-HIV-2LTR
Plasmid#104590Purposestandard for qPCRDepositorInsert2LTR circle junction of HIV-1
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
EcMBP165-cpGFP.PPYF.T203V.pRSET
Plasmid#33373Purposea single-wavelength maltose sensor constructed from E. coli MalE and cpGFPDepositorInsertImproved MBP based Maltose Biosensor
UseTags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted between AA1…PromoterT7Available sinceDec. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pG-HIV-LRT
Plasmid#104592PurposeStandard for qPCRDepositorInsertHIV-1 late reverse transcripts
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21
Plasmid#105522PurposeT7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC1-REX-YFP
Plasmid#48247PurposeGenetically encoded fluorescent indicator for measuring NAD/NADH ratio.DepositorInsertREX-YFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMusheLL
Plasmid#47023PurposeMouse PV L1 and L2, Bicistronic, too large to self-packageDepositorInsertMu L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEDA5_GFPmut3_Y66H
Plasmid#80085PurposePositive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.DepositorInsertGFPmut3_Y66H
UseTags6x-His tagExpressionBacterialMutationchanged Tyrosine 66 to HistidinePromoterproBAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-loxSTOPlox-LynEGFP
Plasmid#133922PurposeReporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombinationDepositorInsertLynEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-Blast
Plasmid#109334PurposepInducer20 with added blasticidin resistanceDepositorTypeEmpty backboneUseLentiviral; Inducible expressionTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
CLIMP-63 pcDNA
Plasmid#80977Purposemammalian expression of CLIMP-63DepositorInsertCLIMP-63 (CKAP4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX304 Luciferase-V5 blast
Plasmid#98580PurposeLentiviral vector for constitutive expression of Luciferase with C-terminal V5 tagDepositorInsertLuciferase
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p403-BrdU-Inc
Plasmid#71789PurposeBrdU-incorporating plasmid with selectable marker HIS3DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
p405-BrdU-Inc
Plasmid#71791PurposeBrdU-incorporating plasmid with selectable marker LEU2DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAJM.336
Plasmid#108528PurposeLacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.DepositorInsertLacIAM + PTac-YFP
UseTagsExpressionBacterialMutationLacI Q18M A47V F161YPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCS BFP
Plasmid#91757PurposeBacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)DepositorInsertBlue Fluorescent Protein
UseTagsHexa-Histidine, Xpress epitope for detection with…ExpressionBacterialMutationGFP (Y66H)PromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -