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Plasmid#228868PurposePE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequenceDepositorInsertPE7-2A-MLH1-SB-NLS-2A-MLH1-SB
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pTP396
Plasmid#149336PurposeBacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobodyDepositorInsertanti-GFP nanobody
UseTagsBiotin acceptor peptide (Avi-tag), His14, and SUM…ExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-2P-Kv-WPRE (AAV9)
Viral Prep#179463-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-JEDI-2P-Kv-WPRE (#179463). In addition to the viral particles, you will also receive purified pAAV-hSyn-JEDI-2P-Kv-WPRE plasmid DNA. hSyn-driven expression (soma and AIS-localized) of the genetically encoded voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-Jaws-KGC-GFP-ER2 (AAV8)
Viral Prep#65014-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hsyn-Jaws-KGC-GFP-ER2 (#65014). In addition to the viral particles, you will also receive purified pAAV-hsyn-Jaws-KGC-GFP-ER2 plasmid DNA. hSyn-driven Jaws-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFPAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KEAP1_p.R470S
Plasmid#82781PurposeGateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.DepositorInsertKEAP1 (KEAP1 Human)
UseGateway entry vectorTagsExpressionMutationR470S; C624SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHIV-INK4A-mOrange2
Plasmid#110730PurposeMammalian Expression of p16INK4A IRES H2B-mOrange2DepositorInsertp16INK4A IRES H2B-mOrange2 (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-IL2 C125S
Plasmid#104348PurposeExpresses human IL-2 (C125S)DepositorInsertHuman IL-2 (C125S) (IL2 Human)
UseTagsMRGSHHHHHGS-tagExpressionBacterialMutationC125S mutation (TGT -> TCC)PromoterT5Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-lck-jGCaMP8m (AAV9)
Viral Prep#176760-AAV9PurposeReady-to-use AAV9 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8m (#176760). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8m plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGfaABC1DTagsNoneAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LIC1B_Caspase-LOVN7C-7
Plasmid#104629Purposebacterial expression of light-activated caspase-3 with N7C-7 linker variationDepositorInsertCaspase-3 (CASP3 Human)
UseTagsHis and LOV2ExpressionBacterialMutationPromoterT7Available sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPLINTR mCHERRY v1
Pooled Library#179775PurposeSPLINTR (Single-cell Profiling and LINeage TRacing) is a high diversity lentiviral barcode library for tracing clonal lineages using bulk and single-cell readouts.DepositorExpressionMammalianUseLentiviralAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F271-OKT3
Plasmid#190682PurposeEncodes an EGFRvIII-CD3 BITE protein (F271 clone)DepositorInsertAnti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-8xHis-Ub
Plasmid#107392PurposeMammalian expression 8xHis-UbDepositorInsert8 tandem repeats of 6xHis-Ub (See comments below)
UseTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAIP hIL4 co
Plasmid#74169PurposeLentiviral vector for expression of human codon optimized IL4 with puromycin resistance.DepositorInsertIL4 (IL4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32-BAR
Plasmid#126072PurposeFor CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoter35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…Available sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3XFLAG-MKL2
Plasmid#27175PurposeFor mammalian expression of human MKL2 with a 3x FLAG tagDepositorInsertMKL2 (MRTFB Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gsz
Plasmid#109355PurposeGsX chimera with Gαz C-terminusDepositorInsertGsz chimera
UseTagsNoneExpressionMammalianMutationPromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C
Plasmid#105047PurposeOverexpression of APOBEC3C in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only